The use of amplified fragment length polymorphism (AFLP) in the isolation of sex‐specific markers
- 1 April 1999
- journal article
- research article
- Published by Wiley in Molecular Ecology
- Vol. 8 (4), 671-674
- https://doi.org/10.1046/j.1365-294x.1999.00578.x
Abstract
Sex identification is a problem in research and conservation. It can often be solved using a DNA test but this is only an option if a sex‐specific marker is available. Such markers can be identified using the amplified fragment length polymorphism (AFLP) technique. This is usually a taxonomic method, as it produces a DNA fingerprint of 50–100 PCR bands. However, if male and female AFLP products are compared, sex‐specific markers are confined to the heterogametic sex and can rapidly be identified. Once a marker is found, AFLP can be used to sex organisms directly or the marker can be sequenced and a standard PCR test designed.Keywords
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