Electrochemical Biosensor Based on Surface Imprinting for Zika Virus Detection in Serum
- 31 December 2018
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Sensors
- Vol. 4 (1), 69-75
- https://doi.org/10.1021/acssensors.8b00885
Abstract
Zika virus (ZIKV) is a flavivirus that was first identified in 1947. Initially, the virus was of little concern for health authorities given there were very few casualties among those suffering an infection. As such, only limited studies were performed on ZIKV. Recently, the viral infection has been linked to microcephaly in infants, which has prompted a dramatic increase in scientific interest in ZIKV research, including methods to allow for rapid virus identification. In this work we report the development of a new type of ZIKV electrochemical biosensor based on surface imprinted polymers and graphene oxide composites. The biosensor was used to detect ZIKV by measuring changes in the electrical signal with changing virus concentrations in buffer and serum using standard electrochemical techniques. The detection limit of our method is similar to the detection limit of the real-time quantitative reverse transcription PCR method.Keywords
Funding Information
- Thailand Research Fund (PHD/0170/2556)
- Kasetsart University Research and Development Institute
- Thailand Government
This publication has 30 references indexed in Scilit:
- Rapid Detection of Zika Virus in Urine Samples and Infected Mosquitos by Reverse Transcription-Loop-Mediated Isothermal AmplificationScientific Reports, 2018
- Aptamer-Based ELISA Assay for Highly Specific and Sensitive Detection of Zika NS1 ProteinAnalytical Chemistry, 2017
- An Update on Zika Virus in AsiaInfection & Chemotherapy, 2017
- Zika Virus Infection in Pregnant Women in Rio de JaneiroThe New England Journal of Medicine, 2016
- Zika and the Risk of MicrocephalyThe New England Journal of Medicine, 2016
- Zika virus, vectors, reservoirs, amplifying hosts, and their potential to spread worldwide: what we know and what we should investigate urgentlyInternational Journal of Infectious Diseases, 2016
- The global spread of Zika virus: is public and media concern justified in regions currently unaffected?Infectious Diseases of Poverty, 2016
- Association between Zika virus and microcephaly in French Polynesia, 2013–15: a retrospective studyThe Lancet, 2016
- Concern over Zika virus grips the worldThe Lancet, 2016
- Zika Virus (I). Isolations and serological specificityTransactions of the Royal Society of Tropical Medicine and Hygiene, 1952