Mild Posttraumatic Hypothermia Reduces Mortality after Severe Controlled Cortical Impact in Rats

Abstract

The effect of posttraumatic hypothermia (brain temperature controlled at 32°C for 4 h) on mortality after severe controlled cortical impact (CCI) was studied in rats. Four posttraumatic brain temperatures were compared: 37°C (n = 10), 36°C (n = 4), 32°C (n = 10), and uncontrolled (UC; n = 6). Rats were anesthetized and subjected to severe CCI (4.0-m/s velocity, 3.0-mm depth) to the exposed left parietal cortex. At 10 min posttrauma the rats were cooled or maintained at their target brain temperature, using external cooling or warming. Brain temperature in the UC group was recorded but not regulated, and rectal temperature was maintained at 37 ± 0.5°C. After 4 h, rats were rewarmed over a 1-h period to 37°C, extubated, and observed for 24 h. In the 37 and 36°C groups, 24-h mortality was 50% (37°C = 5/10, 36°C = 2/4). In the 32°C group, 24-h mortality was 10% (1/10). In the UC group, brain temperature was 35.4 ± 0.6°C during the 4-h treatment period and 24-h mortality was 0% (0/6). Mortality was higher in groups with brain temperatures ≥36°C versus those with brain temperatures