The reaction of lactose with anthrone and its application to the estimation of lactose in casein and other dairy products
- 1 February 1959
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Dairy Research
- Vol. 26 (1), 53-57
- https://doi.org/10.1017/s0022029900009663
Abstract
Lactose was estimated accurately and rapidly in casein by use of anthrone reagent on the clear filtrate from precipitation of a sodium bicarbonate solution of the casein with 0·1 n-sulphuric acid. The reagent consists of 0·15% (w/v) anthrone in 70% (v/v) sulphuric acid. The ice-cold anthrone solution (10 ml.) was added, with cooling, to 1 ml. of the ice-cold solution containing 0–100 µg. of lactose. The mixture was heated at 100° C. for 6 min. and cooled for 30 min. The optical density of the coloured solution was then measured at 625 mµKeywords
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