Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
Open Access
- 20 October 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Cancer
- Vol. 12 (1), 125
- https://doi.org/10.1186/1476-4598-12-125
Abstract
Background The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). Results Herein a two-dimensional gel electrophoresis and ESI-Q-TOF MS/MS-based proteomic approach was used to identify differentially expressed proteins between lung SCC and adjacent normal tissues. 31 proteins with significant alteration were identified. These proteins were mainly involved in metabolism, calcium ion binding, signal transduction and so on. Cathepsin B (CTSB) was one of the most significantly altered proteins and was confirmed by western blotting. Immunohistochemistry showed the correlation between higher CTSB expression and lower survival rate. No statistically significant difference between CTSB-shRNA treated group and the controls was observed in tumor volume, tumor weight, proliferation and apoptosis. However, the CTSB-shRNA significantly inhibited tumor metastases and prolonged survival in LL/2 metastatic model. Moreover, CTSB, Shh and Ptch were up-regulated in patients with metastatic lung SCC, suggesting that hedgehog signaling might be activated in metastatic lung SCC which could affect the expression of CTSB that influence the invasive activity of lung SCC. Conclusions These data suggested that CTSB might serve as a prognostic and therapeutic marker for lung SCC.Keywords
This publication has 27 references indexed in Scilit:
- Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture MicrodissectionJournal of Biomedicine and Biotechnology, 2012
- Transgenic expression of human cathepsin B promotes progression and metastasis of polyoma-middle-T-induced breast cancer in miceOncogene, 2010
- Synergistic antitumor effects of combined cathepsin B and cathepsin Z deficiencies on breast cancer progression and metastasis in miceProceedings of the National Academy of Sciences of the United States of America, 2010
- Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo studyMolecular Cancer, 2010
- Activation of the hedgehog-signaling pathway in human cancer and the clinical implicationsOncogene, 2009
- Identification of metastasis associated proteins in human lung squamous carcinoma using two-dimensional difference gel electrophoresis and laser capture microdissectionLung Cancer, 2009
- Identification of Proteomic Differences between Squamous Cell Carcinoma of the Lung and Bronchial EpitheliumMolecular & Cellular Proteomics, 2009
- Cathepsin B is a target of Hedgehog signaling in pancreatic cancerCancer Letters, 2009
- Applying proteomics to signaling networksCurrent Opinion in Genetics & Development, 2004
- Strategy in Inhibition of Cathepsin B, A Target in Tumor Invasion and MetastasisJournal of the American Chemical Society, 2004