Beyond sequencing: optical mapping of DNA in the age of nanotechnology and nanoscopy
- 31 August 2013
- journal article
- review article
- Published by Elsevier BV in Current Opinion in Biotechnology
- Vol. 24 (4), 690-698
- https://doi.org/10.1016/j.copbio.2013.01.009
Abstract
Next generation sequencing (NGS) is revolutionizing all fields of biological research but it fails to extract the full range of information associated with genetic material. Optical mapping of DNA grants access to genetic and epigenetic information on individual DNA molecules up to ∼1 Mbp in length. Fluorescent labeling of specific sequence motifs, epigenetic marks and other genomic information on individual DNA molecules generates a high content optical barcode along the DNA. By stretching the DNA to a linear configuration this barcode may be directly visualized by fluorescence microscopy. We discuss the advances of these methods in light of recent developments in nano-fabrication and super-resolution optical imaging (nanoscopy) and review the latest achievements of optical mapping in the context of genomic analysis.Keywords
This publication has 44 references indexed in Scilit:
- Denaturation mapping of Saccharomyces cerevisiaeLab on a Chip, 2012
- Single cell genome sequencingCurrent Opinion in Biotechnology, 2012
- A single-step competitive binding assay for mapping of single DNA moleculesBiochemical and Biophysical Research Communications, 2011
- Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNANanoscale, 2011
- Sequence-specific covalent labelling of DNABiochemical Society Transactions, 2011
- Optical mapping of DNA: Single‐molecule‐based methods for mapping genomesPeptide Science, 2011
- Single-molecule denaturation mapping of DNA in nanofluidic channelsProceedings of the National Academy of Sciences of the United States of America, 2010
- High-resolution human genome structure by single-molecule analysisProceedings of the National Academy of Sciences of the United States of America, 2010
- Sequencing technologies — the next generationNature Reviews Genetics, 2009
- Introduction to Molecular Combing: Genomics, DNA Replication, and CancerPublished by Springer Science and Business Media LLC ,2009