Molecular bases of copper and iron deficiency-associated dyslipidemia: a microarray analysis of the rat intestinal transcriptome
- 28 February 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Genes & Nutrition
- Vol. 5 (1), 1-8
- https://doi.org/10.1007/s12263-009-0153-2
Abstract
As essential cofactor in many proteins and redox enzymes, copper and iron are involved in a wide range of biological processes. Mild dietary deficiency of metals represents an underestimated problem for human health, because it does not cause clear signs and clinical symptoms, but it is associated to long-term deleterious effects in cardiovascular system and alterations in lipid metabolism. The aim of this work was to study the biological processes significantly affected by mild dietary deficiency of both metals in rat intestine, in order to better understand the molecular bases of the systemic metabolic alterations, as hypercholesterolemia and hypertriglyceridemia observed in copper-deficient rats. A gene-microarray differential analysis was carried out on the intestinal transcriptome of copper- and iron-deficient rats, thus highlighting the biological processes significantly modulated by the dietary restrictions. The gene array analysis showed a down-regulation of genes involved in mitochondrial and peroxisomal fatty acids beta-oxidation and an up-regulation of genes involved in plasmatic cholesterol transport (apoprotein E and lecithin: cholesterol acyltransferase) in copper deficiency. Furthermore, a severe down-regulation of ApoH was pointed out in iron-deficient animals.Keywords
This publication has 27 references indexed in Scilit:
- How reliable and robust are current biomarkers for copper status?British Journal of Nutrition, 2007
- Copper-binding activity of Trefoil factor 1 (TFF1): A new perspective in the study of the multifunctional roles of TFFsPeptides, 2007
- Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCATBiochemical Journal, 2007
- Intestinal ABCA1 directly contributes to HDL biogenesis in vivoJCI Insight, 2006
- Heart failure improvement from a supplement containing copperEuropean Heart Journal, 2005
- Identification of dietary copper- and iron-regulated genes in rat intestineGene, 2004
- Evidence for inhibition of low density lipoprotein oxidation and cholesterol accumulation by apolipoprotein H (β2-glycoprotein I)Life Sciences, 2001
- Effects of lysolipids and oxidatively modified low density lipoprotein on endothelium-dependent relaxation of rabbit aorta.Circulation Research, 1993
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987
- Studies on the possible function of β2-glycoprotein-I: Influence in the triglyceride metabolism in the ratMetabolism, 1982