Characterization of chloramphenicol-resistant Haemophilus influenzae
- 1 October 1980
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 18 (4), 610-615
- https://doi.org/10.1128/aac.18.4.610
Abstract
We examined nine chloramphenicol-resistant (minimal inhibitory concentration, greater than or equal to 15 micrograms/ml) Haemophilus influenzae strains isolated in various parts of the world to characterize the genetic and biochemical bases of the resistance; four were type b. All nine contained conjugative plasmids, ranging in molecular weight from 34 x 10(6) to 46 x 10(6), which encoded for resistance to chloramphenicol and tetracycline or chloramphenicol, tetracycline, and ampicillin. Deoxyribonucleic acid homology studies showed that these plasmids were closely related to a previously described ampicillin-resistant plasmid, RSF007, and to each other. All nine isolates and their chloramphenicol-resistant transconjugants produced chloramphenicol acetyltransferase. We conclude that chloramphenicol resistance in these strains of H. influenzae is via plasmid-mediated production of chloramphenicol acetyltransferase.This publication has 17 references indexed in Scilit:
- Effect of inoculum size on the susceptibility of Haemophilus influenzae b to beta-lactam antibioticsAntimicrobial Agents and Chemotherapy, 1979
- Characterization and Comparison of Chloramphenicol Acetyltransferase VariantsEuropean Journal of Biochemistry, 1979
- Molecular Properties of Transmissible R Factors of Haemophilus influenzae Determining Tetracycline ResistanceJournal of General Microbiology, 1978
- beta-lactamases and R-plasmids of Haemophilus influenzae.1978
- Molecular Characterization of a Plasmid Specifying Ampicillin Resistance and its Relationship to other R Factors from Haemophilus influenzaeJournal of General Microbiology, 1977
- Plasmid-Mediated Chloramphenicol Resistance in Haemophilus influenzaeAntimicrobial Agents and Chemotherapy, 1977
- Affinity and hydrophobic chromatography of three variants of chloramphenicol acetyltransferases specified by R factors in Escherichia coliFEBS Letters, 1976
- Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmidsJournal of Bacteriology, 1975
- The Enzymatic Acetylation of Chloramphenicol by Extracts of R Factor-resistant Escherichia coliJournal of Biological Chemistry, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951