Phosphorus Removal in an Enhanced Biological Phosphorus Removal Process: Roles of Extracellular Polymeric Substances
- 25 September 2013
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 47 (20), 11482-11489
- https://doi.org/10.1021/es403227p
Abstract
Phosphorus-accumulating organisms are considered to be the key microorganisms in the enhanced biological phosphorus removal (EBPR) process. A large amount of phosphorus is found in the extracellular polymeric substances (EPS) matrix of these microorganisms. However, the roles of EPS in phosphorus removal have not been fully understood. In this study, the phosphorus in the EBPR sludge was fractionated and further analyzed using quantitative (31)P nuclear magnetic resonance spectroscopy. The amounts and forms of phosphorus in EPS as well as their changes in an anaerobic-aerobic process were also investigated. EPS could act as a reservoir for phosphorus in the anaerobic-aerobic process. About 5-9% of phosphorus in sludge was reserved in the EPS at the end of the aerobic phase and might further contribute to the phosphorus removal. The chain length of the intracellular long-chain polyphosphate (polyP) decreased in the anaerobic phase and then recovered under aerobic conditions. However, the polyP in the EPS had a much shorter chain length than the intracellular polyP in the whole cycle. The migration and transformation of various forms of phosphorus among microbial cells, EPS, and bulk liquid were also explored. On the basis of these results, a model with a consideration of the roles of EPS was proposed, which is beneficial to elucidate the mechanism of phosphorus removal in the EBPR system.Keywords
This publication has 33 references indexed in Scilit:
- Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A reviewBiotechnology Advances, 2010
- Relevance of a perchloric acid extraction scheme to determine mineral and organic phosphorus in swine slurryBioresource Technology, 2008
- Biogenic phosphorus in oligotrophic mountain lake sediments: Differences in composition measured with NMR spectroscopyWater Research, 2006
- Characteristics and mechanisms of phosphate adsorption on dewatered alum sludgeSeparation and Purification Technology, 2006
- An Examination of Spin–Lattice Relaxation Times for Analysis of Soil and Manure Extracts by Liquid State Phosphorus‐31 Nuclear Magnetic Resonance SpectroscopyJournal of Environmental Quality, 2006
- Comparison of acetate and propionate uptake by polyphosphate accumulating organisms and glycogen accumulating organismsBiotechnology & Bioengineering, 2005
- The role of extracellular exopolymers in the removal of phosphorus from activated sludgeWater Research, 2001
- Inorganic Polyphosphate: A Molecule of Many FunctionsAnnual Review of Biochemistry, 1999
- Observations relating to enhanced phosphorus removal in biological systemsWater Research, 1992
- 31P-NMR spectroscopy characterization of polyphosphates in activated sludge exhibiting enhanced phosphorus removalWater Research, 1989