The Derivation of Highly Germline-Competent Embryonic Stem Cells Containing NOD-Derived Genome
- 1 January 2003
- journal article
- Published by American Diabetes Association in Diabetes
- Vol. 52 (1), 205-208
- https://doi.org/10.2337/diabetes.52.1.205
Abstract
It would be extremely advantageous to the analysis of disease mechanisms in the spontaneous mouse model of type 1 diabetes, the nonobese diabetic (NOD) strain, if genes in this strain could be modified in vivo using embryonic stem (ES) cells and homologous recombination. However, a NOD ES cell line with adequate germline transmission has not yet been reported. We report the development of highly germline-competent ES cell lines from the F1 hybrid of NOD and 129 for use in NOD gene targeting. Consequently, we developed ES cell lines derived from (NOD × 129)F1 × 129 backcross 1 mice, which were intercrossed to select for homozygosity of particular regions of NOD genome known to contain disease loci.Keywords
This publication has 13 references indexed in Scilit:
- Genetic Protection from the Inflammatory Disease Type 1 Diabetes in Humans and Animal ModelsImmunity, 2001
- CTLA-4−/− Mice Display T Cell-apoptosis Resistance Resembling that Ascribed to Autoimmune-prone Non-obese Diabetic (NOD) MiceJournal of Autoimmunity, 2001
- NOD Idd5 locus controls insulitis and diabetes and overlaps the orthologous CTLA4/IDDM12 and NRAMP1 loci in humans.Diabetes, 2000
- Establishment of an embryonic stem (ES) cell line derived from a non‐obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmissionFEBS Letters, 1999
- Effect of Genetic Background on Establishment of Mouse Embryonic Stem Cells.Experimental Animals, 1999
- I-E+ nonobese diabetic mice develop insulitis and diabetes.The Journal of Experimental Medicine, 1993
- Maternal insulin treatment improves pre-implantation embryo development in diabetic ratsDiabetologia, 1992
- Maternal Diabetes and Retarded Preimplantation Development of MiceDiabetes, 1991
- Maternal diabetes and retarded preimplantation development of miceDiabetes, 1991
- Production of Chimeras by Injecting Cells or Tissue into the BlastocystPublished by Elsevier BV ,1978