Mutation and expression analysis of the putative prostate tumour-suppressor gene PTEN
- 1 November 1998
- journal article
- Published by Springer Science and Business Media LLC in British Journal of Cancer
- Vol. 78 (10), 1296-1300
- https://doi.org/10.1038/bjc.1998.674
Abstract
The chromosomal region 10q23-24 is frequently deleted in a number of tumour types, including prostate adenocarcinoma and glioma. A candidate tumour-suppressor gene at 10q23.3, designated PTENor MMAC1, with putative actin-binding and tyrosine phosphatase domains has recently been described. Mutations in PTEN have been identified in cell lines derived from gliomas, melanomas and prostate tumours and from a number of tumour specimens derived from glial, breast, endometrial and kidney tissue. Germline mutations in PTEN appear to be responsible for Cowden disease. We identified five PTEN mutations in 37 primary prostatic tumours analysed and found that 70% of tumours showed loss or alteration of at least one PTEN allele, supporting the evidence for PTEN involvement in prostate tumour progression. We raised antisera to a peptide from PTEN and showed that reactivity occurs in numerous small cytoplasmic organelles and that the protein is commonly expressed in a variety of cell types. Northern blot analysis revealed multiple RNA species; some arise as a result of alternative polyadenylation sites, but others may be due to alternative splicing.Keywords
This publication has 10 references indexed in Scilit:
- Interfocal heterogeneity of PTEN/MMAC1 gene alterations in multiple metastatic prostate cancer tissues.1998
- Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell linesOncogene, 1998
- MMAC1/PTEN mutations in primary tumor specimens and tumor cell lines.1997
- Frequent inactivation of PTEN/MMAC1 in primary prostate cancer.1997
- Somatic mutations of PTEN in glioblastoma multiforme.1997
- Mutations in PTEN are frequent in endometrial carcinoma but rare in other common gynecological malignancies.1997
- Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in primary breast carcinomas.1997
- Germline mutations in PTEN are present in Bannayan-Zonana syndromeNature Genetics, 1997
- TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta.1997
- PTEN , a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate CancerScience, 1997