Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage‐like cell line
Open Access
- 21 October 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 395 (2-3), 119-122
- https://doi.org/10.1016/0014-5793(96)01015-0
Abstract
Arginase exists in two isoforms. Liver‐type arginase (arginase I) is expressed almost exclusively in the liver and catalyzes the last step of urea synthesis, whereas the nonhepatic type (arginase II) is expressed in extrahepatic tissues. Arginase II has been proposed to play a role in down‐regulation of nitric oxide synthesis. A cDNA for human arginase II was isolated. A polypeptide of 354 amino acid residues including the putative NH2‐terminal presequence for mitochondrial import was predicted. It was 59% identical with arginase I. The arginase II precursor synthesized in vitro was imported into isolated mitochondria and proteolytically processed. mRNA for human arginase II was present in the kidney and other tissues, but was not detected in the liver. Arginase II mRNA was coinduced with nitric oxide synthase mRNA in murine macrophage‐like RAW 264.7 cells by lipopolysaccharide. This induction was enhanced by dexamethasone and dibutyryl cAMP, and was prevented by interferon‐γ. Possible roles of arginase II in NO synthesis are discussed.Keywords
This publication has 28 references indexed in Scilit:
- CCAAT/Enhancer‐Binding Protein β (C/EBPβ) Binds and Activates While Hepatocyte Nuclear Factor‐4 (HNF‐4) does not Bind but Represses the Liver‐Type Arginase PromoterEuropean Journal of Biochemistry, 1996
- Coinduction of Nitric Oxide Synthase, Argininosuccinate Synthetase, and Argininosuccinate Lyase in Lipopolysaccharide-treated RatsPublished by Elsevier BV ,1996
- Co-induction of Arginase and Nitric Oxide Synthase in Murine Macrophages Activated by LipopolysaccharideBiochemical and Biophysical Research Communications, 1995
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991
- Abundance of mRNAs encoding urea cycle enzymes in fetal and neonatal mouse liverArchives of Biochemistry and Biophysics, 1989
- Mitochondrial protein importBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1989
- Isolation of human liver arginase cdna and demonstration of nonhomology between the two human arginase genesBiochemical and Biophysical Research Communications, 1986
- Cloning and expression in Escherichia coli of cDNA for arginase of rat liverBiochemical and Biophysical Research Communications, 1986
- Perinatal development of the liver in rat and spiny mouseEuropean Journal of Biochemistry, 1985
- Permissive Effect of Dexamethasone on Glucagon Induction of Urea‐Cycle Enzymes in Perifused Primary Monolayer Cultures of Rat HepatocytesEuropean Journal of Biochemistry, 1979