A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques
Open Access
- 4 July 2012
- Vol. 12 (7), 9181-9209
- https://doi.org/10.3390/s120709181
Abstract
The success of modern methods in analytical chemistry sometimes obscures the problem that the ever increasing amount of analytical data does not necessarily give more insight of practical relevance. As alternative approaches, toxicity- and bioactivity-based assays can deliver valuable information about biological effects of complex materials in humans, other species or even ecosystems. However, the observed effects often cannot be clearly assigned to specific chemical compounds. In these cases, the establishment of an unambiguous cause-effect relationship is not possible. Effect-directed analysis tries to interconnect instrumental analytical techniques with a biological/biochemical entity, which identifies or isolates substances of biological relevance. Successful application has been demonstrated in many fields, either as proof-of-principle studies or even for complex samples. This review discusses the different approaches, advantages and limitations and finally shows some practical examples. The broad emergence of effect-directed analytical concepts might lead to a true paradigm shift in analytical chemistry, away from ever growing lists of chemical compounds. The connection of biological effects with the identification and quantification of molecular entities leads to relevant answers to many real life questions.Keywords
This publication has 154 references indexed in Scilit:
- Identification strategy for unknown pollutants using high-resolution mass spectrometry: Androgen-disrupting compounds identified through effect-directed analysisAnalytical and Bioanalytical Chemistry, 2011
- Advances in mass spectrometry-based post-column bioaffinity profiling of mixturesAnalytical and Bioanalytical Chemistry, 2010
- Monitoring techniques for odour abatement assessmentWater Research, 2010
- Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assaysAnalytical and Bioanalytical Chemistry, 2010
- Identification of light-independent inhibition of human immunodeficiency virus-1 infection through bioguided fractionation of Hypericum perforatumVirology Journal, 2009
- Reversed-phase liquid chromatography coupled on-line to estrogen receptor bioaffinity detection based on fluorescence polarizationAnalytical and Bioanalytical Chemistry, 2008
- Automated Multiple Ligand Screening by Frontal Affinity Chromatography–Mass Spectrometry (FAC-MS)SLAS Discovery, 2007
- Industrial wastewater analysis: a toxicity-directed approachWaste Management, 1999
- Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionationEnvironmental Science & Technology, 1988
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1975