BMP signaling regulates the dorsal planarian midline and is needed for asymmetric regeneration
Open Access
- 15 November 2007
- journal article
- Published by The Company of Biologists in Development
- Vol. 134 (22), 4043-4051
- https://doi.org/10.1242/dev.007138
Abstract
Planarians can be cut into irregularly shaped fragments capable of regenerating new and complete organisms. Such regenerative capacities involve a robust ability to restore bilateral symmetry. We have identified three genes needed for bilaterally asymmetric fragments to regenerate missing body parts. These genes are candidate components of a signaling pathway that controls the dorsal-ventral patterning of many animal embryos: a BMP1/Tolloid-like gene (smedolloid-1), a SMAD4-like gene (smedsmad4-1), and a BMP2/4/DPP-like gene (smedbmp4-1). BMP signaling was involved in the formation of new tissues at the midline of regeneration, the dorsal-ventral patterning of new tissues, and the maintenance of the dorsal-ventral pattern of existing adult tissue in homeostasis. smedbmp4-1 was normally expressed at the dorsal midline. Asymmetric fragments lacking a midline displayed new smedbmp4-1 expression prior to formation of a regenerative outgrowth (blastema). Asymmetric fragments containing the midline displayed expanded smedbmp4-1 expression towards the wound. We suggest injured animals that lack left-right symmetry reset their midline through modulation of BMP activity as an early and necessary event in regeneration.Keywords
This publication has 32 references indexed in Scilit:
- Bone morphogenetic protein is required for dorso‐ventral patterning in the planarian Dugesia japonicaDevelopment, Growth & Differentiation, 2007
- Regulation of ADMP and BMP2/4/7 at Opposite Embryonic Poles Generates a Self-Regulating Morphogenetic FieldCell, 2005
- SMEDWI-2 Is a PIWI-Like Protein That Regulates Planarian Stem CellsScience, 2005
- The evolution of metazoan axial propertiesNature Reviews Genetics, 2005
- Identification of Genes Needed for Regeneration, Stem Cell Function, and Tissue Homeostasis by Systematic Gene Perturbation in PlanariaDevelopmental Cell, 2005
- New insights into TGF-β–Smad signallingTrends in Biochemical Sciences, 2004
- Molecular Cloning of Bone Morphogenetic Protein (BMP) Gene from the Planarian Dugesia japonicaZoological Science, 1998
- Fish are like flies are like frogs: Conservation of dorsal‐ventral patterning mechanismsBioEssays, 1997
- A transcript from a Drosophila pattern gene predicts a protein homologous to the transforming growth factor-β familyNature, 1987
- The inhibitive action of the Roentgen rays on regeneration in planariansJournal of Experimental Zoology, 1904