A unique residue in rab3c determines the interaction with novel binding protein Zwint‐1
- 14 July 2008
- journal article
- Published by Wiley in FEBS Letters
- Vol. 582 (19), 2838-2842
- https://doi.org/10.1016/j.febslet.2008.07.012
Abstract
Exocytic events are tightly regulated cellular processes in which rab GTPases and their interacting proteins perform an important function. We set out to identify new binding partners of rab3, which mediates regulated secretion events in specialized cells. We discovered Zwint-1 as a rab3 specific binding protein that bound preferentially to rab3c. The interaction depends on a critical residue in rab3c that determines the binding efficiency of Zwint-1, which is immaterial for interaction with rabphilin3a. Rab3c and Zwint-1 are expressed highly in brain and colocalized extensively in primary hippocampal neurons. We also found that SNAP25 bound to the same region in Zwint-1 as rab3c, suggesting a new role for the kinetochore protein Zwint-1 in presynaptic events that are regulated by rab3 and SNAP25.Keywords
This publication has 14 references indexed in Scilit:
- Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpointThe Journal of cell biology, 2008
- RINT-1 Regulates the Localization and Entry of ZW10 to the Syntaxin 18 ComplexMolecular Biology of the Cell, 2006
- Munc13-4 Is an Effector of Rab27a and Controls Secretion of Lysosomes in Hematopoietic CellsMolecular Biology of the Cell, 2005
- A Complete Genetic Analysis of Neuronal Rab3 FunctionJournal of Neuroscience, 2004
- rabip4′ Is an Effector of rab5 and rab4 and Regulates Transport through Early EndosomesMolecular Biology of the Cell, 2004
- Comprehensive Analysis of the Secreted Proteins of the Parasite Haemonchus contortus Reveals Extensive Sequence Variation and Differential Immune RecognitionJournal of Biological Chemistry, 2003
- Localization Versus Function of Rab3 Proteins: EVIDENCE FOR A COMMON REGULATORY ROLE IN CONTROLLING FUSIONPublished by Elsevier BV ,2002
- Comparison of the Effects on Secretion in Chromaffin and PC12 Cells of Rab3 Family Members and MutantsPublished by Elsevier BV ,1999
- Structural Basis of Rab Effector SpecificityCell, 1999
- Association of Rab3A with synaptic vesicles at late stages of the secretory pathway.The Journal of cell biology, 1991