Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot
- 30 November 2004
- journal article
- research article
- Published by Elsevier BV in Clinical Immunology
- Vol. 113 (2), 145-150
- https://doi.org/10.1016/j.clim.2004.07.003
Abstract
Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (n = 20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-CoV. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1—75%, S1-N—25%, S1-C—55%). Evaluation of sera from healthy controls (n = 60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (n = 10) with SARS-like symptoms (ELISA—100% positive, S2 WB—30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-negative SARS cases and in some ELISA-positive non-SARS cases.Keywords
Funding Information
- National Natural Science Foundation of China (30340011)
- Ministry of Education of the People's Republic of China
- Ministry of Science and Technology of the People's Republic of China (2003CB514100)
This publication has 19 references indexed in Scilit:
- Amino Acids 270 to 510 of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Are Required for Interaction with ReceptorJournal of Virology, 2004
- Mounting Lab Accidents Raise SARS FearsScience, 2004
- Profile of Antibodies to the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Probable SARS PatientsClinical and Vaccine Immunology, 2004
- Mosaic Evolution of the Severe Acute Respiratory Syndrome CoronavirusJournal of Virology, 2004
- The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core ComplexJournal of Virology, 2003
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- An Outbreak of Coronavirus OC43 Respiratory Infection in Normandy, FranceClinical Infectious Diseases, 2003
- Molecular Determinants of Species Specificity in the Coronavirus Receptor Aminopeptidase N (CD13): Influence of N-Linked GlycosylationJournal of Virology, 2001
- Serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides andE. coli fusion proteinArchiv für die gesamte Virusforschung, 1995
- Human coronavirus gene expression in the brains of multiple sclerosis patientsVirology, 1992