C-phycocyanin extraction from Spirulina platensis wet biomass
Open Access
- 1 March 2011
- journal article
- Published by FapUNIFESP (SciELO) in Brazilian Journal of Chemical Engineering
- Vol. 28 (1), 45-49
- https://doi.org/10.1590/s0104-66322011000100006
Abstract
C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymatic (lysozyme treatment) methods. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing (the method most frequently used), and presented a extraction yield of 43.75 mg.g-1 and a C-phycocyanin concentration of 0.21 mg.mL-1.Keywords
This publication has 22 references indexed in Scilit:
- C-PHYCOCYANIN EXTRACTION PROCESS FOR LARGE-SCALE USEJournal of Food Biochemistry, 2010
- A novel method of single step hydrophobic interaction chromatography for the purification of phycocyanin from Phormidium fragile and its characterization for antioxidant propertyBioresource Technology, 2008
- Ondas ultrassônicas e pérolas de vidro: um novo método de extração de beta-galactosidase para uso em laboratórioQuímica Nova, 2008
- Optimization of phycocyanin extraction from Spirulina platensis using factorial designBioresource Technology, 2007
- Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentumJournal of Biotechnology, 2002
- Scavenging of Peroxynitrite by Phycocyanin and Phycocyanobilin from Spirulina platensis: Protection against Oxidative Damage to DNABiochemical and Biophysical Research Communications, 2001
- C-Phycocyanin: A Potent Peroxyl Radical Scavenger in Vivo and in VitroBiochemical and Biophysical Research Communications, 2000
- Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyaninProcess Biochemistry, 1999
- Extraction, identification, and quantitation of phycobiliprotein pigments from phototrophic planktonLimnology and Oceanography, 1984
- Cyanobacterial Phycobilisomes. Particles from synechocystis 6701 and two pigment mutantsThe Journal of cell biology, 1980