H3K27 demethylation by JMJD3 at a poised enhancer of anti-apoptotic geneBCL2determines ERα ligand dependency

Abstract

Chromatin represents a repressive barrier to the process of ligand‐dependent transcriptional activity of nuclear receptors. Here, we show that H3K27 methylation imposes ligand‐dependent regulation of the oestrogen receptor α (ERα)‐dependent apoptotic response via Bcl‐2 in breast cancer cells. The activation of BCL2 transcription is dependent on the simultaneous inactivation of the H3K27 methyltransferase, EZH2, and the demethylation of H3K27 at a poised enhancer by the ERα‐dependent recruitment of JMJD3 in hormone‐dependent breast cancer cells. We also provide evidence that this pathway is modified in cells resistant to anti‐oestrogen (AE), which constitutively express BCL2 . We show that the lack of H3K27 methylation at BCL2 regulatory elements due to the inactivation of EZH2 by the HER2 pathway leads to this constitutive activation of BCL2 in these AE‐resistant cells. Our results describe a mechanism in which the epigenetic state of chromatin affects ligand dependency during ERα‐regulated gene expression.