Diagnosis of Creutzfeldt-Jakob Disease by Western Blot Identification of Marker Protein in Human Brain Tissue
- 27 February 1986
- journal article
- research article
- Published by Massachusetts Medical Society in New England Journal of Medicine
- Vol. 314 (9), 547-551
- https://doi.org/10.1056/nejm198602273140904
Abstract
We tested purified preparations of brain tissue from 39 patients with Creutzfeldt–Jakob disease, Gerstmann–Sträussler–Scheinker syndrome, or kuru, and from 32 patients with a variety of nonspongiform degenerative diseases, with the use of Western blots against an antiserum to a similarly purified fraction made from scrapie-infected hamster brain. Positive reactions occurred in 81 percent of the 31 specimens from the patients with Creutzfeldt–Jakob disease (and in all of the 7 specimens that were stored frozen for less than one year), in 3 of the 4 specimens from the patients with kuru, in 3 of the 4 specimens from the patients with Gerstmann–Sträussler–Scheinker syndrome, and in none of the specimens from the patients with other neurologic degenerative disorders, including familial or sporadic Alzheimer's disease; dementia associated with myoclonus, motor neuron disease, or parkinsonism; and acquired-immunodeficiency-syndrome encephalopathy. Immunologic testing has thus begun to provide a useful and rapid adjunct to neuro-pathological examinations and animal-transmission experiments for the diagnosis of the spongiform encephalopathies. (N Engl J Med 1986; 314:547–51.)This publication has 28 references indexed in Scilit:
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