The Structure of the Carbohydrate Backbone of the Lipopolysaccharide from Acinetobacter Strain ATCC 17905

Abstract

The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter strain ATCC 17905 was studied. After deacylation of the lipopolysaccharide, a mixture of two compounds (ratio approximately 2:1) was isolated by high‐performance anion‐exchange chromatography, the structures of which were determined by NMR spectroscopy and electrospray‐mass spectrometry as [Sug, 3‐deoxy‐d‐manno‐2‐octulopyranosonic acid (Kdo) in oligosaccharjde 1 (major portion) and d‐glyc‐ero‐d‐talo‐2‐octulopyranosonic acid (Ko) in oligosaccharide 2 (minor portion)]. All monosaccharide residues also possess the d‐configuration and are present in the pyranose form.