HTII-280, a Biomarker Specific to the Apical Plasma Membrane of Human Lung Alveolar Type II Cells
- 1 October 2010
- journal article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 58 (10), 891-901
- https://doi.org/10.1369/jhc.2010.956433
Abstract
The pulmonary alveolar epithelium is composed of two morphologically distinct cell types, type I (TI) and type II (TII) cells. Alveolar TII cells synthesize, secrete, and recycle surfactant components; contain ion transporters; and secrete immune effector molecules. In response to alveolar injury. TII cells have the capacity to act as progenitor cells, proliferating and transdifferentiating into TI cells. Although various proteins are associated with TII cells, a plasma membrane marker specific to human TII cells that would be useful for identification in tissue and for isolating this cell type has not been described previously. We devised a strategy to produce a monoclonal antibody (MAb) specific to the apical surface of human TII cells and developed an MAb that appears to be specific for human TII cells. The antibody recognizes a 280- to 300-kDa protein, HTII-280, which has the biochemical characteristics of an integral membrane protein. HTII-280 is detected by week 11 of gestation and is developmentally regulated. HTII-280 is useful for isolating human TII cells with purities and viabilities >95%. HTII-280 is likely to be a useful morphological and biochemical marker of human TII cells that may help to advance our understanding of various lung pathological conditions, including the origin and development of various lung tumors.Keywords
This publication has 43 references indexed in Scilit:
- Phenotypes of stem cells from diverse originCytometry Part A, 2009
- Rat alveolar type I cells proliferate, express OCT-4, and exhibit phenotypic plasticity in vitroAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2009
- Rapid urine test for kidney diseaseNature Reviews Nephrology, 2009
- Animal models of acute lung injuryAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2008
- Regulation of surfactant secretion in alveolar type II cellsAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- Coexpression of RTI40 with alveolar epithelial type II cell proteins in lungs following injury: identification of alveolar intermediate cell typesAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2005
- Immunoregulatory functions of surfactant proteinsNature Reviews Immunology, 2005
- Does elevated cardiac troponin I in patients with unstable angina predict ischemia on stress testing?The American Journal of Cardiology, 1999
- Tissue microarrays for high-throughput molecular profiling of tumor specimensNature Medicine, 1998
- Monoclonal antibodies specific to apical surfaces of rat alveolar type I cells bind to surfaces of cultured, but not freshly isolated, type II cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988