Rerouting of Plant Late Endocytic Trafficking Toward a Pathogen Interface
Open Access
- 28 November 2014
- Vol. 16 (2), 204-226
- https://doi.org/10.1111/tra.12245
Abstract
A number of plant pathogenic and symbiotic microbes produce specialized cellular structures that invade host cells where they remain enveloped by host‐derived membranes. The mechanisms underlying the biogenesis and functions of host–microbe interfaces are poorly understood. Here, we show that plant late endocytic trafficking is diverted toward the extrahaustorial membrane (EHM); a host–pathogen interface that develops in plant cells invaded by Irish potato famine pathogen Phytophthora infestans. A late endosome and tonoplast marker protein Rab7 GTPase RabG3c, but not a tonoplast‐localized sucrose transporter, is recruited to the EHM, suggesting specific rerouting of vacuole‐targeted late endosomes to a host–pathogen interface. We revealed the dynamic nature of this process by showing that, upon activation, a cell surface immune receptor traffics toward the haustorial interface. Our work provides insight into the biogenesis of the EHM and reveals dynamic processes that recruit membrane compartments and immune receptors to this host–pathogen interface.Keywords
Funding Information
- Gatsby Charitable Foundation
- European Research Council
- Biotechnology and Biological Sciences Research Council
- Marie Curie Reintegration Grant (Grant FP7-PEOPLE-2009-RG (reference no 268419))
- the Swiss National Science Foundation grant (PBFRP3-137182)
This publication has 81 references indexed in Scilit:
- Filamentous plant pathogen effectors in actionNature Reviews Microbiology, 2013
- Cellular programs for arbuscular mycorrhizal symbiosisCurrent Opinion in Plant Biology, 2012
- Visualizing Cellular Dynamics in Plant–Microbe Interactions Using Fluorescent-Tagged ProteinsPublished by Springer Science and Business Media LLC ,2011
- Intracellular plant microbe associations: secretory pathways and the formation of perimicrobial compartmentsCurrent Opinion in Plant Biology, 2010
- Recent advances in rice blast effector researchCurrent Opinion in Plant Biology, 2010
- Plant immunity: towards an integrated view of plant–pathogen interactionsNature Reviews Genetics, 2010
- Trafficking vesicles: pro or contra pathogens?Current Opinion in Plant Biology, 2009
- Terrific Protein Traffic: The Mystery of Effector Protein Delivery by Filamentous Plant PathogensScience, 2009
- Cell Wall–Associated Mechanisms of Disease Resistance and SusceptibilityAnnual Review of Phytopathology, 2007
- Tête à tête inside a plant cell: establishing compatibility between plants and biotrophic fungi and oomycetesNew Phytologist, 2006