Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses.
- 1 December 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 83 (24), 9759-9763
- https://doi.org/10.1073/pnas.83.24.9759
Abstract
To investigate whether DNA viruses can augment gene expression of the human immunodeficiency virus (HIV), cotransfection experiments were carried out in which a recombinant plasmid containing the HIV long terminal repeat (LTR) linked to the chloramphenicol acetyltransferase (CAT) gene was transfected into cultured cells along with plasmids containing DNA from various distinct classes of DNA viruses. Molecular clones containing JC virus, BK virus, lymphotropic papovavirus, bovine papilloma virus, type 1 herpes simplex virus (HSV-1), and varicella-zoster virus sequences increased CAT expression directed by the HIV LTR. Trans-activation of the HIV LTR varied in different cell lines, but in each case the HIV tat gene product elicited the greatest stimulation. Primer-extension assays specific for HIV LTR mRNA revealed increased levels of steady-state RNA following transfection with HIV tat as well as with several of the DNA viruses. Virus-specific RNA expression paralleled the stimulation of CAT activity. More-than-additive effects were observed at both the RNA and protein levels when tat plus type 1 herpes simplex virus DNAs or tat plus JC virus DNAs were transfected into cells with the HIV LTR-CAT plasmid. These data suggest that coinfection of cells by HIV and some DNA viruses can stimulate the expression of HIV.Keywords
This publication has 31 references indexed in Scilit:
- Post-transcriptional regulation accounts for the trans-activation of the human T-lymphotropic virus type IIINature, 1986
- Genomic Heterogeneity of AIDS Retroviral Isolates from North America and ZaireScience, 1985
- Functional Relation Between HTLV-II x and Adenovirus E1A Proteins in Transcriptional ActivationScience, 1985
- Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene productCell, 1985
- Isolation of Lymphocytopathic Retroviruses from San Francisco Patients with AIDSScience, 1984
- Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDSScience, 1984
- Activation of gene expression by adenovirus and herpesvirus regulatory genes acting in trans and by a cis-acting adenovirus enhancer elementCell, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979