Interaction of enteroaggregative Escherichia coli with salad leaves
- 20 July 2009
- journal article
- Published by Wiley in Environmental Microbiology Reports
- Vol. 1 (4), 234-239
- https://doi.org/10.1111/j.1758-2229.2009.00037.x
Abstract
Enteroaggregative Escherichia coli (EAEC) are important human pathogens. However, their environmental reservoir is unknown. As fresh salad leaves are increasingly recognized as an important environmental vector for human pathogens, we investigated leaf attachment capability of EAEC strains. We found that binding of clinical EAEC isolates to leaves from Eruca vesicaria (commonly known as rocket or arugula) can be divided into high, moderate and low adherent phenotypes. Using the prototype EAEC strain 042 to investigate the underlining mechanisms involved in leaf attachment, we found small attached bacterial aggregates over the entire leaf surface and dense bacterial attachment to the guard cell of the stomata. An aaf 042 mutant lost the ability to bind the epidermis while retaining stomatal adherence. In contrast, a fliC 042 mutant retained the ability to bind the epidermis but lost stomatal tropism. These results show that multiple adherence factors are involved in the interaction of EAEC with leaves, that EAEC uses similar colonization factors to bind mucosal and leaf surfaces and that fresh produce might be an important reservoir of EAEC strains.Keywords
This publication has 34 references indexed in Scilit:
- Interaction of Salmonella enterica with basil and other salad leavesThe ISME Journal, 2008
- New Adhesin of Enteroaggregative Escherichia coli Related to the Afa/Dr/AAF FamilyInfection and Immunity, 2008
- Enterohemorrhagic Escherichia coli Exploits EspA Filaments for Attachment to Salad LeavesApplied and Environmental Microbiology, 2008
- Detection of enteroaggregative Escherichia coli in faecal samples from patients in the community with diarrhoeaJournal of Medical Microbiology, 2006
- Enteroaggregative Escherichia coli Is a Cause of Acute Diarrheal Illness: A Meta-AnalysisClinical Infectious Diseases, 2006
- Pathogenesis of enteroaggregativeEscherichia coliinfectionFEMS Microbiology Letters, 2006
- Salmonella enterica Virulence Genes Are Required for Bacterial Attachment to Plant TissueApplied and Environmental Microbiology, 2005
- Prevalence of Enteroaggregative Escherichia coli among Children with and without Diarrhea in SwitzerlandJournal of Clinical Microbiology, 2003
- Enteroaggregative Escherichia coli expresses a novel flagellin that causes IL-8 release from intestinal epithelial cellsJCI Insight, 2000
- Heterogeneity of Enteroaggregative Escherichia Coli Virulence DemonstratedThe Journal of Infectious Diseases, 1995