SPARC Is a Key Regulator of Proliferation, Apoptosis and Invasion in Human Ovarian Cancer
Open Access
- 3 August 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (8), e42413
- https://doi.org/10.1371/journal.pone.0042413
Abstract
Secreted protein acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, is implicated in the progression of many cancers. In this study, we investigated the expression and function of SPARC in ovarian cancer. cDNA microarray analysis was performed to compare gene expression profiles of the highly invasive and the low invasive subclones derived from the SKOV3 human ovarian cancer cell line. Immunohistochemistry (IHC) staining was performed to investigate SPARC expression in a total of 140 ovarian tissue specimens. In functional assays, effects of SPARC knockdown on the biological behavior of ovarian cancer cells were investigated. The mechanisms of SPARC in ovarian cancer proliferation, apoptosis and invasion were also researched. SPARC was overexpressed in the highly invasive subclone compared with the low invasive subclone. High SPARC expression was associated with high stage, low differentiation, lymph node metastasis and poor prognosis of ovarian cancer. Knockdown of SPARC expression significantly suppressed ovarian cancer cell proliferation, induced cell apoptosis and inhibited cell invasion and metastasis. SPARC is overexpressed in highly invasive subclone and ovarian cancer tissues and plays an important role in ovarian cancer growth, apoptosis and metastasis.Keywords
This publication has 43 references indexed in Scilit:
- The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapyCell Death & Differentiation, 2011
- The p53/p21Cip1/ Waf1 pathway mediates the effects of SPARC on melanoma cell cycle progressionPigment Cell & Melanoma Research, 2010
- Relationship and prognostic significance of SPARC and VEGF protein expression in colon cancerJournal of Experimental & Clinical Cancer Research, 2010
- Downregulation of SPARC expression decreases gastric cancer cellular invasion and survivalJournal of Experimental & Clinical Cancer Research, 2010
- The biology of ovarian cancer: new opportunities for translationNature Reviews Cancer, 2009
- Integrin β3 down-regulates invasive features of ovarian cancer cells in SKOV3 cell subclonesZeitschrift für Krebsforschung und Klinische Onkologie, 2008
- SPARC upregulates MT1-MMP expression, MMP-2 activation, and the secretion and cleavage of galectin-3 in U87MG glioma cellsNeuroscience Letters, 2007
- Frequent inactivation of SPARC by promoter hypermethylation in colon cancersInternational Journal of Cancer, 2007
- Secreted Protein Acidic and Rich in Cysteine (SPARC) Inhibits Integrin-Mediated Adhesion and Growth Factor-Dependent Survival Signaling in Ovarian CancerThe American Journal of Pathology, 2007
- Bone-related Genes Expressed in Advanced Malignancies Induce Invasion and Metastasis in a Genetically Defined Human Cancer ModelPublished by Elsevier BV ,2003