Inhibition of CK2 binding to BMPRIa induces C2C12 differentiation into osteoblasts and adipocytes
- 1 May 2013
- journal article
- research article
- Published by Wiley in Journal of Cell Communication and Signaling
- Vol. 7 (4), 265-278
- https://doi.org/10.1007/s12079-013-0199-1
Abstract
BMP2 is a growth factor that regulates the cell fate of mesenchymal stem cells into osteoblast and adipocytes. However, the detailed signaling pathways and mechanism are unknown. We previously reported a new interaction of Casein kinase II (CK2) with the BMP receptor type-Ia (BMPRIa) and demonstrated using mimetic peptides CK2.1, CK2.2 and CK2.3 that the release of CK2 from BMPRIa activates Smad signaling and osteogenesis. Previously, we showed that mutation of these CK2 sites on BMPRIa (MCK2.1 (476S-A), MCK2.2 (324S-A) and MCK2.3 (214S-A)) induced osteogenesis. However, one mutant MCK2.1 induced osteogenesis similar to overexpression of wild type BMPRIa, suggesting that the effect of this mutant on mineralization was due to overexpression. In this paper we investigated the signaling pathways involved in the CK2-BMPRIa mediated osteogenesis and identified a new signaling pathway activating adipogenesis dependent on the BMPRIa and CK2 association. Further the mechanism for adipogenesis and osteogenesis is specific to the CK2 interaction site on BMPRIa. In detail our data show that overexpression of MCK2.2 induced osteogenesis was dependent on Caveolin-1 (Cav1) and the activation of the Smad and mTor pathways, while overexpression of MCK2.3 induced osteogenesis was independent of Caveolin-1 without activation of Smad pathway. However, MCK2.3 induced osteogenesis via the MEK pathway. The adipogenesis induced by the overexpression of MCK2.2 in C2C12 cells was dependent on the p38 and ERK pathways as well as Caveolin-1. These data suggest that signaling through BMPRIa used two different signaling pathways to induce osteogenesis dependent on CK2. Additionally the data supports a signaling pathway initiated in caveolae and one outside of caveolae to induce mineralization. Moreover, they reveal the signaling pathway of BMPRIa mediated adipogenesis.Keywords
This publication has 42 references indexed in Scilit:
- BMP2-activated Erk/MAP Kinase Stabilizes Runx2 by Increasing p300 Levels and Histone Acetyltransferase ActivityOnline Journal of Public Health Informatics, 2010
- Casein Kinase 2 β-Subunit Is a Regulator of Bone Morphogenetic Protein 2 SignalingBiophysical Journal, 2010
- Transforming Growth Factor-β1 Induces Transdifferentiation of Myoblasts into Myofibroblasts via Up-Regulation of Sphingosine Kinase-1/S1P3 AxisMolecular Biology of the Cell, 2010
- Emerging role of bone morphogenetic proteins in adipogenesis and energy metabolismCytokine & Growth Factor Reviews, 2009
- FRET Reveals Novel Protein-Receptor Interaction of Bone Morphogenetic Proteins Receptors and Adaptor Protein 2 at the Cell SurfaceBiophysical Journal, 2009
- Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stressJournal of Neurochemistry, 2009
- BMP2 Regulates Osterix through Msx2 and Runx2 during Osteoblast DifferentiationJournal of Biological Chemistry, 2008
- PRDM16 controls a brown fat/skeletal muscle switchNature, 2008
- Transcriptional Control of Brown Fat Determination by PRDM16Cell Metabolism, 2007
- Bone Morphogenetic ProteinsGrowth Factors, 2004