Coupled pre‐mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli
Open Access
- 1 January 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Systems Biology
- Vol. 7 (1), 529
- https://doi.org/10.1038/msb.2011.62
Abstract
Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time‐dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre‐mRNA) and mature mRNA profiles. Transcriptome‐wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time‐dependent degradation, shaping transient responses with precise timing and amplitude.Keywords
This publication has 52 references indexed in Scilit:
- The highways and byways of mRNA decayNature Reviews Molecular Cell Biology, 2007
- The abundance of the spliceosomal snRNPs is not limiting the splicing of U12-type intronsRNA, 2006
- Regulating Gene Expression through RNA Nuclear RetentionCell, 2005
- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem CellsCell, 2005
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- RNA polymerase II is an essential mRNA polyadenylation factorNature, 1998
- Feedback Inhibition of Macrophage Tumor Necrosis Factor-α Production by TristetraprolinScience, 1998
- Transcriptional autoregulation of the proto-oncogene fosNature, 1988
- Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.Proceedings of the National Academy of Sciences of the United States of America, 1987
- The potential for the formation of a biosynthetic enzyme in Escherichia coliBiochimica et Biophysica Acta, 1957