hPOC5 is a centrin-binding protein required for assembly of full-length centrioles
Open Access
- 6 April 2009
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 185 (1), 101-114
- https://doi.org/10.1083/jcb.200808082
Abstract
Centrin has been shown to be involved in centrosome biogenesis in a variety of eukaryotes. In this study, we characterize hPOC5, a conserved centrin-binding protein that contains Sfi1p-like repeats. hPOC5 is localized, like centrin, in the distal portion of human centrioles. hPOC5 recruitment to procentrioles occurs during G2/M, a process that continues up to the full maturation of the centriole during the next cell cycle and is correlated with hyperphosphorylation of the protein. In the absence of hPOC5, RPE1 cells arrest in G1 phase, whereas HeLa cells show an extended S phase followed by cell death. We show that hPOC5 is not required for the initiation of procentriole assembly but is essential for building the distal half of centrioles. Interestingly, the hPOC5 family reveals an evolutionary divergence between vertebrates and organisms like Drosophila melanogaster or Caenorhabditis elegans, in which the loss of hPOC5 may correlate with the conspicuous differences in centriolar structure.Keywords
This publication has 62 references indexed in Scilit:
- Control of daughter centriole formation by the pericentriolar materialNature, 2008
- The Uni2 Phosphoprotein is a Cell Cycle–regulated Component of the Basal Body Maturation Pathway inChlamydomonas reinhardtiiMolecular Biology of the Cell, 2008
- Plk4-Induced Centriole Biogenesis in Human CellsDevelopmental Cell, 2007
- Centrosome biogenesis and function: centrosomics brings new understandingNature Reviews Molecular Cell Biology, 2007
- Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cellsThe Journal of cell biology, 2007
- Centriole assembly in Caenorhabditis elegansNature, 2006
- Inhibition of centrosome protein assembly leads to p53-dependent exit from the cell cycleThe Journal of cell biology, 2006
- Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary ciliaNature, 2005
- Proteomic characterization of the human centrosome by protein correlation profilingNature, 2003
- Toward a functional analysis of the yeast genome through exhaustive two-hybrid screensNature Genetics, 1997