Nanoporous CREG-Eluting Stent Attenuates In-Stent Neointimal Formation in Porcine Coronary Arteries
Open Access
- 3 April 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (4), e60735
- https://doi.org/10.1371/journal.pone.0060735
Abstract
The goal of this study was to evaluate the efficacy of a nanoporous CREG-eluting stent (CREGES) in inhibiting neointimal formation in a porcine coronary model. In vitro proliferation assays were performed using isolated human endothelial and smooth muscle cells to investigate the cell-specific pharmacokinetic effects of CREG and sirolimus. We implanted CREGES, control sirolimus-eluting stents (SES) or bare metal stents (BMS) into pig coronary arteries. Histology and immunohistochemistry were performed to assess the efficacy of CREGES in inhibiting neointimal formation. CREG and sirolimus inhibited in vitro vascular smooth muscle cell proliferation to a similar degree. Interestingly, human endothelial cell proliferation was only significantly inhibited by sirolimus and was increased by CREG. CREGES attenuated neointimal formation after 4 weeks in porcine coronary model compared with BMS. No differences were found in the injury and inflammation scores among the groups. Scanning electron microscopy and CD31 staining by immunohistochemistry demonstrated an accelerated reendothelialization in the CREGES group compared with the SES or BMS control groups. The current study suggests that CREGES reduces neointimal formation, promotes reendothelialization in porcine coronary stent model.This publication has 33 references indexed in Scilit:
- Cellular repressor of E1A-stimulated genes regulates vascular endothelial cell migration by The ILK/AKT/mTOR/VEGF165 signaling pathwayExperimental Cell Research, 2011
- Glycosylation-independent binding to extracellular domains 11–13 of mannose-6-phosphate/insulin-like growth factor-2 receptor mediates the effects of soluble CREG on the phenotypic modulation of vascular smooth muscle cellsJournal of Molecular and Cellular Cardiology, 2011
- CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosisExperimental Cell Research, 2009
- Secreted CREG inhibits cell proliferation mediated by mannose 6‐phosphate/insulin‐like growth factor II receptor in NIH3T3 fibroblastsGenes to Cells, 2008
- CREG promotes a mature smooth muscle cell phenotype and reduces neointimal formation in balloon-injured rat carotid arteryCardiovascular Research, 2008
- Percutaneous coronary interventions with drug eluting stents for diabetic patientsHeart, 2005
- Differential Response of Delayed Healing and Persistent Inflammation at Sites of Overlapping Sirolimus- or Paclitaxel-Eluting StentsCirculation, 2005
- Localized Hypersensitivity and Late Coronary Thrombosis Secondary to a Sirolimus-Eluting StentCirculation, 2004
- Oral Everolimus Inhibits In-Stent Neointimal GrowthCirculation, 2002
- Assessing performances of lodogen-coated surfaces used for radioiodination of proteinsNuclear Medicine Communications, 1990