Immobilization of Enzyme into Poly(vinyl alcohol) Membrane
- 1 November 1986
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 28 (11), 1721-1726
- https://doi.org/10.1002/bit.260281116
Abstract
Glucoamylase, invertase, and cellulase were entrapped within poly(vinyl alcohol) (PVA) membrane cross-linked by means of irradiation of ultraviolet light. The conditions for immobilization of glucoamylase were examined with respect to enzyme concentration in PVA, sensitizer (sodium benzoate) concentration in PVA, irradiation time, and membrane thickness. Various characteristics of immobilized glucoamylase were evaluated. Among them, the pH activity curve for the immobilized enzyme was superior to that for the native one, and thermal stability was improved by immobilization with bovine albumin. The apparent Km was larger for immobilized glucoamylase than for the native one, while Vmax was smaller for the immobilized enzyme. Also, the apparent Km appeared to be affected by the molecular size of the substrate. Further, immobilized invertase and cellulase showed good stabilities in repeating usage.Keywords
This publication has 5 references indexed in Scilit:
- Preparation of immobilized invertase using poly(vinyl alcohol) membraneBiotechnology & Bioengineering, 1983
- Studies on the immobilized enzymes by radiopolymerization. IV. Preparation of membranous immobilized invertase and it's characteristics.Agricultural and Biological Chemistry, 1976
- Preparation of immobilized enzymes using poly(vinyl alcohol)Biotechnology & Bioengineering, 1973
- New methods for binding enzyme molecules into a water‐insoluble matrix: Properties after insolubilizationBiotechnology & Bioengineering, 1973
- Preparation and General Properties of Glucoamylase Bound to Halogenacetyl CelluloseAgricultural and Biological Chemistry, 1972