Microvalve Enabled Digital Microfluidic Systems for High-Performance Biochemical and Genetic Analysis
Open Access
- 1 December 2010
- journal article
- other
- Published by SAGE Publications in SLAS Technology
- Vol. 15 (6), 455-463
- https://doi.org/10.1016/j.jala.2010.08.003
Abstract
Microfluidic devices offer unparalleled capability for digital microfluidic automation of sample processing and complex assay protocols in medical diagnostic and research applications. In our own work, monolithic membrane valves have enabled the creation of two platforms that precisely manipulate discrete, nanoliter-scale volumes of sample. The digital microfluidic automaton uses two-dimensional microvalve arrays to combinatorially process nanoliter-scale sample volumes. This programmable system enables rapid integration of diverse assay protocols using a universal processing architecture. Microfluidic emulsion generator array (MEGA) devices integrate actively controlled three microvalve pumps to enable on-demand generation of uniform droplets for statistical encapsulation of microbeads and cells. A MEGA device containing 96 channels confers the capability of generating up to 3.4 × 106-nL volume droplets per hour for ultrahigh-throughput detection of rare mutations in a vast background of normal genotypes. These novel digital microfluidic platforms offer significant enhancements in throughput, sensitivity, and programmability for automated sample processing and analysis.Keywords
This publication has 26 references indexed in Scilit:
- A digital microfluidic platform for the automation of quantitative biomolecular assaysLab on a Chip, 2009
- A Digital Microfluidic Approach to Homogeneous Enzyme AssaysAnalytical Chemistry, 2008
- Droplet microfluidicsLab on a Chip, 2008
- A fully integrated microfluidic genetic analysis system with sample-in–answer-out capabilityProceedings of the National Academy of Sciences of the United States of America, 2006
- Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencingProceedings of the National Academy of Sciences of the United States of America, 2006
- A review of microvalvesJournal of Micromechanics and Microengineering, 2006
- An integrated microfluidic processor for single nucleotide polymorphism-based DNA computingLab on a Chip, 2005
- Droplet-based microfluidic lab-on-a-chip for glucose detectionAnalytica Chimica Acta, 2004
- Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devicesSensors and Actuators B: Chemical, 2003
- Microfluidic Large-Scale IntegrationScience, 2002