Iron deficiency induces sulfate uptake and modulates redistribution of reduced sulfur pool in barley plants
- 1 January 2006
- journal article
- Published by CSIRO Publishing in Functional Plant Biology
- Vol. 33 (11), 1055-1061
- https://doi.org/10.1071/fp06179
Abstract
We studied the possibility that the sulfur (S) assimilatory pathway might be modulated by iron (Fe) starvation in barley, as a consequence of plant requirement for an adequate amount of reduced S to maintain methionine and, in turn, phytosiderophore biosynthesis. Barley seedlings were grown with or without 100 µm FeIII–EDTA, at three S levels in the nutrient solution (S2 = 1200, S1 = 60, and S0 = 0 µm sulfate) in order to reproduce conditions of optimal supply, latent and severe deficiency, respectively. Fe deprivation increased root cysteine content irrespective of the S supply. However, this increase was not associated with either higher rates of 35SO42– uptake or increased expression of the gene for the high-affinity sulfate transporter, HvST1, and these roots failed to increase their activities of ATP sulfurylase (ATPS) and O-acetylserine(thiol) lyase (OASTL). We observed a significant increase in 35SO42– uptake rate (+76%) only in Fe-deficient S1 plants and we found an increase in root ATPS activity only in S0 plants. We observed an increase of ATPS enzyme activity in leaves of S1 and S2 plants, most likely suggesting increased S assimilation followed by translocation of thiols (Cys) to the root. Taken together, our results suggest that Fe deficiency affects the partitioning from the shoot to the root of the reduced S pool within the plant and can affect SO42– uptake under limited S supply.Keywords
This publication has 26 references indexed in Scilit:
- Sulfur starvation reduces phytosiderophores release by iron-deficient barley plantsSoil Science and Plant Nutrition, 2006
- Regulation of Sulfate Uptake and Expression of Sulfate Transporter Genes in Brassica oleracea as Affected by Atmospheric H2S and Pedospheric Sulfate NutritionPlant Physiology, 2004
- Influence of iron nutrition on sulphur uptake and metabolism in maize (Zea maysL.) rootsSoil Science and Plant Nutrition, 2004
- Does the Sulfur Assimilation Pathway Play a Role in the Response to Fe Deficiency in Maize (Zea mays L.) Plants?Journal of Plant Nutrition, 2003
- Leaf Responses of Young Iron‐Inefficient Maize Plants to Sulfur DeprivationJournal of Plant Nutrition, 2003
- Transporter gene families in plants: the sulphate transporter gene family — redundancy or specialization?Physiologia Plantarum, 2003
- Molecular genetics of sulphate assimilationAdvances in Botanical Research, 2000
- Phytochelatins in Cadmium-Sensitive and Cadmium-Tolerant Silene vulgaris (Chain Length Distribution and Sulfide Incorporation)Plant Physiology, 1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Determination of Reserve Sulfur and Soluble Sulfates in SoilsSoil Science Society of America Journal, 1960