In vitro studies on a two-pool storage of adrenaline and noradrenaline in granule material from bovine adrenal medulla
- 1 August 1980
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 109 (4), 345-354
- https://doi.org/10.1111/j.1748-1716.1980.tb06606.x
Abstract
Adrenal medullary granules were isolated by millipore filtration according to Poisner & Trifaró (1967) and then lysed in deionized water. In a perfusion system the influence of concentration of pH was studied on the uptake of biogenic amines (PhEA, TA, DA, NA, A, Arch and Hi) and sodium ions by lysed and by dialyzed material. The results suggest a two-pool storage of A and NA in the granules. A minor pool with cation exchanger properties binds unselectively organic (biogenic amines) and inorganic cations with a Umax of 400-500 nmol/mg granules dry-weight. This minor pool (pool 1) was fully charged at amine concentrations greater than 10 mM. A larger pool binds selectively A and NA--possibly stereospecifically L-NA and L-A--with a Umax approximately 1500 nmol/mg granules. This larger pool (pool 2) required A and NA concentrations approximately 200-300 mM for maximal filling. In pool 2 CA+ ions are assumed to be electrostatically linked to carboxyl groups, which become available as hypothetical COO----+H3N salt bridges are successively forced open by increasing CA concentrations (greater than 10-30 mM). ATP- ions become attached to the concomitantly unmasked +NH3 groups.Keywords
This publication has 8 references indexed in Scilit:
- The Ability of ATP‐Free Granule Material from Bovine Adrenal Medulla to Bind Inorganic Cations and Biogenic AminesActa Physiologica Scandinavica, 1977
- Anin vitro-Formed Protamine-Heparin Complex as a Model for a Two-Compartment Store for Biogenic AminesActa Physiologica Scandinavica, 1976
- Storage of biogenic amines in blood platelets and adrenal medullaNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1972
- A possible mechanism for uptake of biogenic amines by storage organelles: Incorporation into nucleotide-metal aggregatesCellular and Molecular Life Sciences, 1971
- Binding of Some Biogenic Amines in TissuesAdvances in Pharmacology, 1962
- Further Observations on the State of the Catechol Amines Stored in the Adrenal Medullary GranulesActa Physiologica Scandinavica, 1960
- Content and Intracellular Distribution of Adenosine Triphosphate in Cow Adrenal Medulla1Acta Physiologica Scandinavica, 1956
- Observations on the distribution of catechol amines and adenosinetriphosphate in the bovine adrenal medullaThe Journal of Physiology, 1956