An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells
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- 14 August 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 29 (9), 829-834
- https://doi.org/10.1038/nbt.1947
Abstract
Using cells derived from human pluripotent stem cells for therapeutic applications carries a risk that rare undifferentiated cells in the transplant will give rise to teratomas. Tang et al. identify a new antigen on the surface of human pluripotent cells that, combined with other antigens, enables complete depletion of teratoma-initiating cells. An important risk in the clinical application of human pluripotent stem cells (hPSCs), including human embryonic and induced pluripotent stem cells (hESCs and hiPSCs), is teratoma formation by residual undifferentiated cells. We raised a monoclonal antibody against hESCs, designated anti–stage-specific embryonic antigen (SSEA)-5, which binds a previously unidentified antigen highly and specifically expressed on hPSCs—the H type-1 glycan. Separation based on SSEA-5 expression through fluorescence-activated cell sorting (FACS) greatly reduced teratoma-formation potential of heterogeneously differentiated cultures. To ensure complete removal of teratoma-forming cells, we identified additional pluripotency surface markers (PSMs) exhibiting a large dynamic expression range during differentiation: CD9, CD30, CD50, CD90 and CD200. Immunohistochemistry studies of human fetal tissues and bioinformatics analysis of a microarray database revealed that concurrent expression of these markers is both common and specific to hPSCs. Immunodepletion with antibodies against SSEA-5 and two additional PSMs completely removed teratoma-formation potential from incompletely differentiated hESC cultures.Keywords
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