Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA
- 1 March 1998
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 392 (6671), 42-48
- https://doi.org/10.1038/32100
Abstract
The nuclear factor of activated T cells (NFAT) and the AP-1 heterodimer, Fos–Jun, cooperatively bind a composite DNA site and synergistically activate the expression of many immune-response genes. A 2.7-Å-resolution crystal structure of the DNA-binding domains of NFAT, Fos and Jun, in a quaternary complex with a DNA fragment containing the distal antigen-receptor response element from the interleukin-2 gene promoter, shows an extended interface between NFAT and AP-1, facilitated by the bending of Fos and DNA. The tight association of the three proteins on DNA creates a continuous groove for the recognition of 15 base pairs.Keywords
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