Controlling the spatial distribution of ECM components in degradable PEG hydrogels for tissue engineering cartilage
- 1 November 2002
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 64A (1), 70-79
- https://doi.org/10.1002/jbm.a.10319
Abstract
In developing a scaffold to support new tissue growth, the degradation rate and mass loss profiles of the scaffold are important design parameters. In this study, hydrogels were prepared by copolymerizing a degradable macromer, poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) endcapped with acrylate groups (PEG-LA-DA) with a nondegradable macromer, poly(ethylene glycol) dimethacrylate (PEGDM). The resulting hydrogels exhibited a range of degradation behavior and mass loss profiles. Chondrocytes were photoencapsulated in gels formulated with 50:50, 25:75, and 15:85 (mol % PEGDM: mol % PEG-LA-DA) and cultured for 6 weeks in vitro. The neocartilaginous tissue formed was examined biochemically and histologically. After 6 weeks, the DNA content in gels with 75 and 85% degradable crosslinks was nearly twice that of the DNA content in the 50% gels. The total collagen content was significantly higher in the 85% gel [2.4 ± 0.8% wet weight (ww)] compared to the 50% gel (0.22 ± 0.29% ww). In examining the neocartilaginous tissue with immunohistochemistry, type II collagen was localized in the pericellular region in the 50% gel; however, when increased degradation was incorporated into the gel, type II collagen was found throughout the neotissue. In summary, the important role of hydrogel degradation in controlling and influencing the deposition and distribution of extracellular matrix molecules was demonstrated and quantified. © 2002 Wiley Periodicals, Inc., J Biomed Mater Res 64A: 70–79, 2003Keywords
This publication has 26 references indexed in Scilit:
- A Statistical Kinetic Model for the Bulk Degradation of PLA-b-PEG-b-PLA Hydrogel Networks: Incorporating Network Non-IdealitiesThe Journal of Physical Chemistry B, 2001
- A Generalized Bulk-Degradation Model for Hydrogel Networks Formed from Multivinyl Cross-linking MoleculesThe Journal of Physical Chemistry B, 2001
- Synthesis and degradation of a tri-component copolymer derived from glycolide, L-lactide, and ε-caprolactoneJournal of Biomaterials Science, Polymer Edition, 2000
- Cartilage reconstruction in head and neck surgery: Comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilageJournal of Biomedical Materials Research, 1998
- New Technique to Extend the Useful Life of a Biodegradable Cartilage ImplantTissue Engineering, 1998
- The Effects of Porosity on in Vitro Degradation of Polylactic Acid–Polyglycolic Acid Implants Used in Repair of Articular CartilageTissue Engineering, 1998
- Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay.JCI Insight, 1994
- Preparation and characterization of poly(l-lactic acid) foamsPolymer, 1994
- Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromersMacromolecules, 1993
- Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymersJournal of Biomedical Materials Research, 1993