Programming gene expression with combinatorial promoters
Open Access
- 1 January 2007
- journal article
- Published by Springer Science and Business Media LLC in Molecular Systems Biology
- Vol. 3 (1), 145
- https://doi.org/10.1038/msb4100187
Abstract
Promoters control the expression of genes in response to one or more transcription factors (TFs). The architecture of a promoter is the arrangement and type of binding sites within it. To understand natural genetic circuits and to design promoters for synthetic biology, it is essential to understand the relationship between promoter function and architecture. We constructed a combinatorial library of random promoter architectures. We characterized 288 promoters in Escherichia coli, each containing up to three inputs from four different TFs. The library design allowed for multiple −10 and −35 boxes, and we observed varied promoter strength over five decades. To further analyze the functional repertoire, we defined a representation of promoter function in terms of regulatory range, logic type, and symmetry. Using these results, we identified heuristic rules for programming gene expression with combinatorial promoters.Keywords
This publication has 51 references indexed in Scilit:
- A synthetic phage λ regulatory circuitProceedings of the National Academy of Sciences of the United States of America, 2006
- Gene Expression From Random Libraries of Yeast PromotersGenetics, 2006
- Plasticity of the cis-Regulatory Input Function of a GenePLoS Biology, 2006
- Transcriptional Regulation by Competing Transcription Factor ModulesPLoS Computational Biology, 2006
- Bacterial Repression Loops Require Enhanced DNA FlexibilityJournal of Molecular Biology, 2005
- Engineered gene circuitsNature, 2002
- Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coliGene, 1994
- Synergistic Activation of Transcription by Bacteriophage λ cI Protein and E. coli cAMP Receptor ProteinScience, 1994
- Recognition of nucleotide sequences at the Escherichia coli galactose operon P1 promoter by RNA polymeraseGene, 1989
- Genetic regulatory mechanisms in the synthesis of proteinsJournal of Molecular Biology, 1961