Inhibition by Ca2+ of inositol trisphosphate-mediated Ca2+ liberation: a possible mechanism for oscillatory release of Ca2+.
- 1 January 1990
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 87 (1), 260-264
- https://doi.org/10.1073/pnas.87.1.260
Abstract
Light-flash photolysis of caged inositol 1,4,5-trisphosphate (InsP3) was used to generate reproducible transients of free InsP3 in Xenopus oocytes, and the resulting liberation of Ca2+ from intracellular stores was monitored by recording Ca2+-activated membrane currents and by use of the fluorescent Ca2+ indicator fluo-3. InsP3-mediated Ca2+ release was inhibited by elevating the intracellular free Ca2+ level, either by microinjecting Ca2+ into the cell or by applying conditioning light flashes to liberate Ca2+. This inhibition followed a slow time course, being maximal after about 2 s and subsequently declining over several seconds. Negative feedback of Ca2+ ions on InsP3-mediated Ca2+ liberation may explain the oscillatory release of Ca2+ seen during activation of inositol phospholipid signaling in the oocyte, and the time course of the inhibition is consistent with the period of the oscillations.Keywords
This publication has 29 references indexed in Scilit:
- Ionic and charge-displacement currents evoked by temperature jumps in Xenopus oocytesProceedings of the Royal Society of London. B. Biological Sciences, 1989
- Properties and Uses of Photoreactive Caged CompoundsAnnual Review of Biophysics and Biophysical Chemistry, 1989
- Inositol trisphosphate‐induced membrane potential oscillations in Xenopus oocytes.The Journal of Physiology, 1988
- Injection of inositol 1, 3, 4, 5-tetrakisphosphate into Xenopus oocytes generates a chloride current dependent upon intracellular calciumProceedings of the Royal Society of London. B. Biological Sciences, 1987
- Crosslinkage of B lymphocyte surface immunoglobulin by anti-Ig or antigen induces prolonged oscillation of intracellular ionized calcium.The Journal of Experimental Medicine, 1987
- INOSITOL TRISPHOSPHATE AND DIACYLGLYCEROL: TWO INTERACTING SECOND MESSENGERSAnnual Review of Biochemistry, 1987
- Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphateNature, 1987
- Oscillatory chloride current evoked by temperature jumps during muscarinic and serotonergic activation in Xenopus oocyte.The Journal of Physiology, 1987
- Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytesProceedings of the Royal Society of London. B. Biological Sciences, 1983
- Simulation of intracellular Ca2+ oscillation in a sympathetic neuroneJournal of Theoretical Biology, 1981