Production and characterization of a monoclonal antibody that cross-reacts with the mycotoxins nivalenol and 4-deoxynivalenol
- 1 August 2006
- journal article
- research article
- Published by Taylor & Francis Ltd in Food Additives & Contaminants
- Vol. 23 (8), 816-825
- https://doi.org/10.1080/02652030600699072
Abstract
Nivalenol is a mycotoxin produced by certain fungi that are pathogenic to important cereal crops, in particular maize, wheat, and barley. This toxin, 3α,4β,7α,15-tetrahydroxy-12,13-epoxytrichothec-9-en-8-one, is found worldwide and is closely related to 4-deoxynivalenol (DON or vomitoxin), a mycotoxin associated with outbreaks of Fusarium head blight in North America. The literature on the toxicity of nivalenol suggests it is similar, if not more toxic, than DON. Despite the development of rapid immunologically based assays for detecting DON, such assays have not existed for detecting nivalenol without chemical modification of the analyte. This paper describes the development of a monoclonal antibody using a nivalenol–glycine protein conjugate. The monoclonal antibody was most specific for an acetylated form of DON (3-Ac-DON), but it exhibited sensitivity and cross-reactivity that were useful for detecting nivalenol and DON at relevant levels without the need to modify either toxin chemically. In an competitive indirect ELISA format, the concentrations of toxins able to inhibit colour development by 50% (IC50) were 1.7, 15.8, 27.5, 68.9, and 1740 ng ml−1 for the mycotoxins 3-Ac-DON, DON, nivalenol, 15-Ac-DON, and fusarenon-X, respectively. The antibody was also used to develop a competitive direct ELISA for DON and nivalenol, with IC50's of 16.5 ng ml−1 (DON) and 33.4 ng ml−1 (nivalenol). These assays are capable of detecting both DON and nivalenol simultaneously, a property that may be useful in regions where these toxins co-occur or in formats, such as immunoaffinity columns, where co-isolation of both toxins is desirable.Keywords
This publication has 31 references indexed in Scilit:
- Survey of Fusarium toxins in foodstuffs of plant origin marketed in GermanyInternational Journal of Food Microbiology, 2005
- Rapid methods for deoxynivalenol and other trichothecenesToxicology Letters, 2004
- Co-occurrence of Fusarium mycotoxins in mouldy and healthy corn from KoreaFood Additives & Contaminants, 1999
- Detection of Acetylated Deoxynivalenol by Enzyme-Linked Immunosorbent AssayPublished by American Chemical Society (ACS) ,1996
- Natural occurrence of acetylated derivatives of deoxynivalenol and nivalenol in wheat and barley in JapanFood Additives & Contaminants, 1995
- Production of Monoclonal Antibodies for the Specific Detection of Deoxynivalenol and 15-Acetyldeoxynivalenol by ELISAJournal of Agricultural and Food Chemistry, 1995
- Production of a monoclonal antibody with specificity for deoxynivalenol, 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenolFood and Agricultural Immunology, 1993
- An enzyme‐linked immunosorbent assay for deoxynivalenol in wheat, utilizing novel hapten derivatization proceduresFood and Agricultural Immunology, 1990
- Preparation and characterization of polyclonal and monoclonal antibodies against fusarenon‐XFood and Agricultural Immunology, 1989
- Enzyme-linked immunosorbent assay employing monoclonal antibody specific for deoxynivalenol (vomitoxin) and several analogsJournal of Agricultural and Food Chemistry, 1988