Capillary electrophoresis‐mass spectrometry‐based metabolome analysis of serum and saliva from neurodegenerative dementia patients
- 6 September 2013
- journal article
- Published by Wiley in Electrophoresis
- Vol. 34 (19), 2865-2872
- https://doi.org/10.1002/elps.201300019
Abstract
Despite increasing global prevalence, the precise pathogenesis and terms for objective diagnosis of neurodegenerative dementias remain controversial, and comprehensive understanding of the disease remains lacking. Here, we conducted metabolomic analysis of serum and saliva obtained from patients with neurodegenerative dementias (n = 10), including Alzheimer's disease, frontotemporal lobe dementia, and Lewy body disease, as well as from age‐matched healthy controls (n = 9). Using CE‐TOF‐MS, six metabolites in serum (β‐alanine, creatinine, hydroxyproline, glutamine, iso‐citrate, and cytidine) and two in saliva (arginine and tyrosine) were significantly different between dementias and controls. Using multivariate analysis, serum was confirmed as a more efficient biological fluid for diagnosis compared to saliva; additionally, 45 metabolites in total were identified as candidate markers that could discriminate at least one pair of diagnostic groups from the healthy control group. These metabolites possibly provide an objective method for diagnosing dementia‐type by multiphase screening. Moreover, diagnostic‐type‐dependent differences were observed in several tricarboxylic acid cycle compounds detected in serum, indicating that some pathways in glucose metabolism may be altered in dementia patients. This pilot study revealed novel alterations in metabolomic profiles between various neurodegenerative dementias, which would contribute to etiological investigations.Keywords
This publication has 38 references indexed in Scilit:
- Epidemiology of Alzheimer diseaseNature Reviews Neurology, 2011
- Anti-Aβ Therapeutics in Alzheimer's Disease: The Need for a Paradigm ShiftNeuron, 2011
- Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profilesMetabolomics, 2009
- Metabolomic mapping of atypical antipsychotic effects in schizophreniaMolecular Psychiatry, 2007
- TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosisBiochemical and Biophysical Research Communications, 2006
- Ubiquitinated TDP-43 in Frontotemporal Lobar Degeneration and Amyotrophic Lateral SclerosisScience, 2006
- Association between CSF biomarkers and incipient Alzheimer's disease in patients with mild cognitive impairment: a follow-up studyThe Lancet Neurology, 2006
- Capillary electrophoresis‐mass spectrometry in food analysisElectrophoresis, 2005
- Alzheimer's Disease: The Amyloid Cascade HypothesisScience, 1992
- Clinical diagnosis of Alzheimer's diseaseNeurology, 1984