Variation in the cellular localization of host‐protective oncospheral antigens inTaenia saginataandTaenia solium
- 4 August 2010
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 32 (9-10), 684-695
- https://doi.org/10.1111/j.1365-3024.2010.01230.x
Abstract
Immunohistochemistry and immunofluorescence with confocal microscopy were used to localize the host‐protective antigens of Taenia saginata (TSA9 and TSA18) and Taenia solium (TSOL16, TSOL18 and TSOL45). In nonactivated oncospheres, TSA9 and TSOL45 antigens were found primarily in the cytoplasm of the penetration gland type one (PG1) cell. A similar pattern of staining was seen for TSOL45 in oncospheres of T. solium that remained within the oncospheral membrane. In addition, there was less intense staining of TSA9 and TSOL45 in the quadri‐nucleate penetration gland type 2 (PG2) cell. TSA18, TSOL16 and TSOL18 were predominantly found in the PG2 cell. In activated oncospheres that had escaped the oncospheral membrane, the antigens (other than TSA9) were seen both in the penetration gland cell locations and throughout the oncospheral parenchyma. Co‐localization analyses revealed that only TSOL16 and TSOL18 antigens were co‐localized in the PG2 cell of oncospheres that had not escaped the oncospheral membrane. However, in activated oncospheres that escaped the oncospheral membrane, all three antigens of T. solium were co‐localized as they were present throughout the parenchyma. No positive staining was observed on the surface of nonactivated or recently activated oncospheres of T. saginata or T. solium.This publication has 34 references indexed in Scilit:
- Ultrastructural reconstruction of Taenia ovis oncospheres from serial sectionsInternational Journal for Parasitology, 2010
- Localisation of three host-protective oncospheral antigens of Taenia ovisInternational Journal for Parasitology, 2010
- Elimination of Taenia solium transmission to pigs in a field trial of the TSOL18 vaccine in CameroonInternational Journal for Parasitology, 2010
- The ultrastructure of taeniid cestode oncospheres and localization of host-protective antigensParasitology, 2009
- IN VITRO ONCOSPHERE-KILLING ASSAYS TO DETERMINE IMMUNITY TO THE LARVAE OF TAENIA PISIFORMIS, TAENIA OVIS, TAENIA SAGINATA, AND TAENIA SOLIUMJournal of Parasitology, 2006
- Vaccination Against Cysticercosis and Hydatid DiseaseParasitology Today, 2000
- An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding proteinGene, 1988
- Passive protection against Taenia saginata infection in cattle by a mouse monoclonal antibody reactive with the surface of the invasive oncosphereParasite Immunology, 1986
- Echinococcus granulosus: Hook-muscle systems and cellular organisation of infective oncospheresInternational Journal for Parasitology, 1983
- STUDIES ON IMMUNE RESPONSES TO LARVAL CESTODES IN MICE. INCREASED SUSCEPTIBILITY OF CERTAIN MOUSE STRAINS AND HYPOTHYMIC MICE TO TAENlA TAENIAEFORMIS AND ANALYSIS OF PASSIVE TRANSFER OF RESISTANCE WITH SERUMImmunology & Cell Biology, 1977