Metabolomics Analysis Reveals the Compositional Differences of Shade Grown Tea (Camellia sinensis L.)
- 8 December 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (1), 418-426
- https://doi.org/10.1021/jf902929h
Abstract
The different cultivation methods affect tea quality by altering the basic metabolite profiles. In this study, the metabolome changes were investigated in green tea and shade cultured green tea (tencha) by liquid chromatography−mass spectrometry (LC-MS) and gas chromatography−mass spectrometry (GC-MS) coupled with a multivariate data set. The principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) of green tea clearly showed higher levels of galloylquinic acid, epigallocatechin, epicatechin, succinic acid, and fructose, together with lower levels of gallocatechin, strictinin, apigenin glucosyl arabinoside, quercetin p-coumaroylglucosyl-rhamnosylgalactoside, kaempferol p-coumaroylglucosylrhamnosylgalactoside, malic acid, and pyroglutamic acid than tencha. The effects of some seasonal variations were also observed in the primary metabolite concentrations such as amino acids and organic acids. In addition, green tea showed stronger antioxidant activity than tencha in both April and July. The antioxidant activity of green tea samples were significantly correlated with their total phenol and total flavonoid contents. This present study delineates the possibility to get high umami and less astringent green teas in shade culture. It highlights the metabolomic approaches to find out the effect of cultivation methods on chemical composition in plants and the relationship with antioxidant activity.Keywords
This publication has 31 references indexed in Scilit:
- Study on discrimination of Roast green tea (Camellia sinensis L.) according to geographical origin by FT-NIR spectroscopy and supervised pattern recognitionSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2009
- Ultra‐performance LC/TOF MS analysis of medicinal Panax herbs for metabolomic researchJournal of Separation Science, 2008
- Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escaroleFood Chemistry, 2007
- Possible mechanisms of the cancer‐preventive activities of green teaMolecular Nutrition & Food Research, 2006
- Mass spectrometry in metabolome analysisMass Spectrometry Reviews, 2004
- Metabolite Profiling Using 1H NMR Spectroscopy for Quality Assessment of Green Tea, Camellia sinensis (L.)Journal of Agricultural and Food Chemistry, 2004
- Simultaneous determination of catechins, caffeine and other phenolic compounds in tea using new HPLC methodJournal of Food Composition and Analysis, 2004
- Antioxidant Activity of Tea Polyphenols In Vivo: Evidence from Animal StudiesJournal of Nutrition, 2003
- Identification of Chlorophylls and Carotenoids in Major Teas by High-Performance Liquid Chromatography with Photodiode Array DetectionJournal of Agricultural and Food Chemistry, 2003
- Protection against Nitric Oxide Toxicity by TeaJournal of Agricultural and Food Chemistry, 2000