Evaluation of a modified HET-CAM assay as a screening test for eye irritancy
- 31 August 1996
- journal article
- Published by Elsevier BV in Toxicology in Vitro
- Vol. 10 (4), 431-446
- https://doi.org/10.1016/0887-2333(96)00021-5
Abstract
The hen's egg test-chorioallantoic membrane (HET-CAM) assay, an alternative to the Draize eye irritation test, was developed by Luepke and has been improved on by means of a microscopic examination and the use of a test substance applicator (TSA). The TSA is a double teflon ring in which a perlon mesh is locked, and has several advantages over conventional protocols, reducing subjectivity of the method and avoiding the need for rinsing after treatment. It was confirmed by statistical analysis that the HET-CAM-TSA method can reproduce potentialin vivo irritant effects on the conjunctiva. The classification based on thein vitro results was compared with fourin vivo classifications [MAS (maximal average score) with thresholds of 15.0 and 25.0; the Kay and Calandra method; and EC criteria]. Cooper's parameters (specificity, sensitivity and concordance with the Draize test) were calculated according to these fourin vivo classifications. When the most rigorous classification (MAS threshold of 15.0) was taken into account, a sensitivity of 80%, a specificity of 81.3% and a concordance with the Draize test of 80.4% were obtained for this set of 46 compounds.Keywords
This publication has 17 references indexed in Scilit:
- The EC/HO international validation study on alternatives to the draize eye irritation testToxicology in Vitro, 1995
- Comparison of five potential methods for assessing ocular irritation in vitroToxicology in Vitro, 1995
- Interlaboratory assessment of the bovine corneal opacity and permeability (BCOP) assayToxicology in Vitro, 1994
- Recent Progress in the Eye Irritation TestToxicology and Industrial Health, 1993
- Criteria for in vitro alternatives for the eye irritation testFood and Chemical Toxicology, 1993
- An evaluation of five potential alternatives in vitro to the rabbit eye irritation test in vivoToxicology in Vitro, 1992
- Bovine corneal opacity and permeability test: An in vitro assay of ocular irritancy*1Fundamental and Applied Toxicology, 1992
- Correlation and validation of alternative methods to the Draize eye irritation test (OPAL project)Toxicology in Vitro, 1991
- Describing the validity of carcinogen screening testsBritish Journal of Cancer, 1979
- Chemical Burns of the Rabbit Cornea*American Journal of Ophthalmology, 1946