Seizure-like activity in hyaluronidase-treated dissociated hippocampal cultures
Open Access
- 1 January 2013
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cellular Neuroscience
- Vol. 7, 58373
- https://doi.org/10.3389/fncel.2013.00149
Abstract
The extracellular matrix (ECM) plays an important role in use-dependent synaptic plasticity. Hyaluronic acid (HA) is the backbone of the neural ECM, which has been shown to modulate -amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA) receptor mobility, paired-pulse depression, L-type voltage-dependent Ca2+ channel (L-VDCC) activity, long-term potentiation and contextual fear conditioning. To investigate the role of HA in the development of spontaneous neuronal network activity, we used microelectrode array recording and Ca2+ imaging in hippocampal cultures enzymatically treated with hyaluronidase. Our findings revealed an appearance of epileptiform activity 9 days after hyaluronidase treatment. The treatment transformed the normal network firing bursts and Ca2+ oscillations into long-lasting “superbursts” and “superoscillations” with durations of 11-100 seconds. The changes in Ca2+ transients in hyaluronidase-treated neurons were more prominent then in astrocytes and preceded changes in electrical activity. The Ca2+ superoscillations could be suppressed by applying the L-VDCC blocker diltiazem, whereas the neuronal firing superbursts could be additionally suppressed by an 6-cyano-7-nitroquinoxaline-2,3-dione as an antagonist of AMPA/kainate receptors. These results suggest that changes in the expression of HA can be epileptogenic and that hyaluronidase treatment in vitro provides a robust model for the dissection of the underlying mechanisms.This publication has 28 references indexed in Scilit:
- Perineuronal net formation and structure in aggrecan knockout miceNeuroscience, 2010
- Self-organization and neuronal avalanches in networks of dissociated cortical neuronsNeuroscience, 2008
- Recurrent seizures and brain pathology after inhibition of glutamine synthetase in the hippocampus in ratsBrain, 2008
- The role of chondroitin sulfate proteoglycans in regeneration and plasticity in the central nervous systemBrain Research Reviews, 2007
- An extremely rich repertoire of bursting patterns during the development of cortical culturesBMC Neuroscience, 2006
- Seizure-Induced Plasticity of h Channels in Entorhinal Cortical Layer III Pyramidal NeuronsNeuron, 2004
- Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivoNature Methods, 2004
- Unsupervised Spike Detection and Sorting with Wavelets and Superparamagnetic ClusteringNeural Computation, 2004
- Acquired Dendritic Channelopathy in Temporal Lobe EpilepsyScience, 2004
- Postnatal development of perineuronal nets in wild-type mice and in a mutant deficient in tenascin-RJournal of Comparative Neurology, 2000