MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells
- 7 April 2011
- journal article
- Published by Wiley in FEBS Letters
- Vol. 585 (9), 1322-1330
- https://doi.org/10.1016/j.febslet.2011.03.067
Abstract
RAC1 regulates a diverse array of cellular events including migration and invasion. MicroRNAs (miRNAs) have a key role in the regulation of gene expression. In this study, we demonstrated that microRNA-142-3p (miR-142-3p) acted as a negative regulator of human RAC1. Overexpression of miR-142-3p decreased RAC1 mRNA and protein levels. Moreover, the overexpression of miR-142-3p suppressed, while blocking of miR-142-3p increased colony formation, migration and invasion in hepatocellular carcinoma (HCC) cell lines (QGY-7703 and SMMC-7721). RAC1 overexpression without the 3′untranslated region abolished the effect of miR-142-3p in the QGY-7703 and SMMC-7721 cells. These results demonstrated that miR-142-3p directly and negatively regulates RAC1 in HCC cells, which highlights the importance of miRNAs in tumorigenesisKeywords
Funding Information
- National Natural Science Foundation of China (30873017, 91029714, 31071191)
- Natural Science Foundation of Tianjin (08JCZDJC23300, 09JCZDJC17500)
This publication has 28 references indexed in Scilit:
- MicroRNA-130b regulates the tumour suppressor RUNX3 in gastric cancerEuropean Journal of Cancer, 2010
- Novel aspects of the roles of Rac1 GTPase in the cardiovascular systemCurrent Opinion in Pharmacology, 2010
- Regulation of the transcription factor NF-κB1 by microRNA-9 in human gastric adenocarcinomaMolecular Cancer, 2010
- MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitinCancer Letters, 2009
- Activation of the small GTPase Rac1 by a specific guanine‐nucleotide‐exchange factor suffices to induce glucose uptake into skeletal‐muscle cellsBiology of the Cell, 2008
- Oncomirs — microRNAs with a role in cancerNature Reviews Cancer, 2006
- Short RNAs Repress Translation after Initiation in Mammalian CellsMolecular Cell, 2006
- The global health burden of infection‐associated cancers in the year 2002International Journal of Cancer, 2006
- A microRNA polycistron as a potential human oncogeneNature, 2005
- Cross-talk between Rac1 GTPase and dysregulated Wnt signaling pathway leads to cellular redistribution of β-catenin and TCF/LEF-mediated transcriptional activationOncogene, 2004