Alterations of Regional Cerebral Blood Flow and Oxygen Saturation in a Rat Sinus-Vein Thrombosis Model
- 1 April 1996
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Stroke
- Vol. 27 (4), 720-728
- https://doi.org/10.1161/01.str.27.4.720
Abstract
Background and Purpose The pathophysiology of sinus-vein thrombosis (SVT) in patients and experimental animals is still poorly understood. This study was designed to examine and further elucidate the pathophysiological sequence of events, especially the relationship between local and regional blood flow and hemoglobin oxygen saturation (HbSO 2 ) detected at identical locations. The use of both parameters as outcome indicators should be compared. Methods SVT was induced by ligation of the superior sagittal sinus (SSS) and slow injection of kaolin-cephalin suspension into the SSS in rats. Regional cerebral blood flow (rCBF) was assessed by laser-Doppler flowmetry together with regional HbSO 2 , which was measured by a microspectrophotometric technique at 48 identical locations for 90 minutes after SVT using a scanning technique. Fluorescence angiography was performed before and 30 and 90 minutes after SVT induction. After 48 hours the animals were killed for histology. Results The fluorescence angiographic findings could divide animals into three groups: (1) group A, with a solitary SSS thrombus (n=8); (2) group B, with a thrombosis of SSS and cortical veins (n=10); (3) group C, animals that had undergone sham operation (n=5). Decreases of rCBF and HbSO 2 and brain damage were seen in group B but not in group A. The reduction of local HbSO 2 preceded the flow decrease after sagittal sinus ligation but before thrombosis. Blood pressure in group A was found to be significantly higher after SVT than in groups B and C. Conclusions The brain with acute extension of a thrombus from the SSS into cortical veins experiences a critically reduced supply of blood and oxygen. CBF, local HbSO 2 , and repeated angiography can be helpful monitors for the early detection of critical conditions after SVT. Local HbSO 2 has a greater sensitivity to predict outcome than lCBF. Moreover, therapies directed to improve perfusion pressure or reduce vascular resistance may open further therapeutic windows during SVT progression.Keywords
This publication has 16 references indexed in Scilit:
- Use of Local Cerebral Blood Flow Monitoring to Predict Brain Damage after Disturbance to the Venous CirculationNeurosurgery, 1995
- Cerebral Blood Flow Autoregulation during Hypobaric Hypotension Assessed by Laser Doppler ScanningJournal of Cerebral Blood Flow & Metabolism, 1994
- Effect of arterial PCO2on local HbO2and relative Hb concentration in the human brain-a study with the Erlangen micro-lightguide spectrophotometer (EMPHO)Physiological Measurement, 1994
- Neuropathological changes following occlusion of the superior sagittal sinus and cerebral veins in the catNeuropathology and Applied Neurobiology, 1994
- Intravital Microreflectometry of Individual Pial Vessels and Capillary Region of RatJournal of Cerebral Blood Flow & Metabolism, 1994
- Effect of Cervical Sympathectomy and Hypoxia on the Heterogeneity of O2 Saturation of Small Cerebrocortical VeinsJournal of Cerebral Blood Flow & Metabolism, 1993
- Loss of cerebral regulation during cardiac output variations in focal cerebral ischemiaJournal of Neurosurgery, 1992
- Occlusion of the pig superior sagittal sinus, bridging and cortical veins: multistep evolution of sinus-vein thrombosisJournal of Neurosurgery, 1992
- The Erlangen micro-lightguide spectrophotometer EMPHO IPhysics in Medicine & Biology, 1989
- Experiments on Thrombosis of the Superior Longitudinal SinusJournal of Neurosurgery, 1946