Requirement for Generation of H 2 O 2 for Platelet-Derived Growth Factor Signal Transduction
Open Access
- 13 October 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 270 (5234), 296-299
- https://doi.org/10.1126/science.270.5234.296
Abstract
Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.Keywords
This publication has 31 references indexed in Scilit:
- Differential Effects of Platelet-derived Growth Factor BB on p125 Focal Adhesion Kinase and Paxillin Tyrosine Phosphorylation and on Cell Migration in Rabbit Aortic Vascular Smooth Muscle Cells and Swiss 3T3 FibroblastsOnline Journal of Public Health Informatics, 1995
- Integrins and Signal Transduction Pathways: the Road TakenScience, 1995
- H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance responseCell, 1994
- Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.The Journal of cell biology, 1994
- Viral, worm and radical implications for apoptosisTrends in Biochemical Sciences, 1994
- Bcl-2 functions in an antioxidant pathway to prevent apoptosisCell, 1993
- The pathogenesis of atherosclerosis: a perspective for the 1990sNature, 1993
- Selective inhibition of protein tyrosine phosphatase activities by H2O2 and vanadate In vitroBiochemical and Biophysical Research Communications, 1992
- Dietary Antioxidants and Cardiovascular DiseaseAnnals of the New York Academy of Sciences, 1992
- Signal Transduction by the Platelet-Derived Growth Factor ReceptorScience, 1989