ATP stimulates secretion in human neutrophils and HL60 cells via a pertussis toxin‐sensitive guanine nucleotide‐binding protein coupled to phospholipase C

Abstract

Human neutrophils and HL60 cells respond to extracellular ATP by causing exocytotic secretion. Secretion is accompanied by increases in inositol phosphates and a rise in cytosol Ca²⁺. The responses to ATP are blocked by pertussis toxin pretreatment, indicating the involvement of a guanine nucleotide regulatory protein. Other nucleotides that are active in promoting secretion are ATPγS, UTP, ITP and AppNHp, whilst 8-bromo-ATP, AppCH₂p, ADP, AMP and adenosine are inactive.