STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria
- 15 May 2013
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 110 (22), 8936-8941
- https://doi.org/10.1073/pnas.1301820110
Abstract
The mitochondrial inner membrane organizing system (MINOS) is a conserved large hetero-oligomeric protein complex in the mitochondrial inner membrane, crucial for the maintenance of cristae morphology. MINOS has been suggested to represent the core of an extended protein network that controls mitochondrial function and structure, and has been linked to several human diseases. The spatial arrangement of MINOS within mitochondria is ill-defined, however. Using super-resolution stimulated emission depletion (STED) microscopy and immunogold electron microscopy, we determined the distribution of three known human MINOS subunits (mitofilin, MINOS1, and CHCHD3) in mammalian cells. Super-resolution microscopy revealed that all three subunits form similar clusters within mitochondria, and that MINOS is more abundant in mitochondria around the nucleus than in peripheral mitochondria. At the submitochondrial level, mitofilin, a core MINOS subunit, is preferentially localized at cristae junctions. In primary human fibroblasts, mitofilin labeling uncovered a regularly spaced pattern of clusters arranged in parallel to the cell growth surfaces. We suggest that this array of MINOS complexes might explain the observed phenomenon of largely horizontally arranged cristae junctions that connect the inner boundary membrane to lamellar cristae. The super-resolution images demonstrate an unexpectedly high level of regularity in the nanoscale distribution of the MINOS complex in human mitochondria, supporting an integrating role of MINOS in the structural organization of the organelle.Keywords
This publication has 41 references indexed in Scilit:
- Mitofilin complexes: conserved organizers of mitochondrial membrane architectureBiological Chemistry, 2012
- Endoplasmic reticulum–mitochondria contacts: function of the junctionNature Reviews Molecular Cell Biology, 2012
- Role of MINOS in Mitochondrial Membrane Architecture: Cristae Morphology and Outer Membrane Interactions Differentially Depend on Mitofilin DomainsJournal of Molecular Biology, 2012
- The mitochondrial contact site complex, a determinant of mitochondrial architectureThe EMBO Journal, 2011
- Sample Preparation for STED MicroscopyPublished by Springer Science and Business Media LLC ,2009
- Computationally Driven, Quantitative Experiments Discover Genes Required for Mitochondrial BiogenesisPLoS Genetics, 2009
- The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled‐coil‐helix coiled‐coil‐helix domain‐containing protein 3 and 6 and DnaJC11FEBS Letters, 2007
- Mitochondrial Morphology and Dynamics in Yeast and Multicellular EukaryotesAnnual Review of Genetics, 2005
- The cristal membrane of mitochondria is the principal site of oxidative phosphorylationFEBS Letters, 2003
- The internal structure of mitochondriaTrends in Biochemical Sciences, 2000