The de novo centriole assembly pathway in HeLa cells
Open Access
- 28 February 2005
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 168 (5), 713-722
- https://doi.org/10.1083/jcb.200411126
Abstract
It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547–1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous “precentrioles” become morphologically recognizable centrioles before mitosis. De novo–assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo–formed centrioles do not mature if they are assembled in S phase–arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.Keywords
This publication has 39 references indexed in Scilit:
- Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplicationThe Journal of cell biology, 2003
- Microtubule release from the centrosome in migrating cellsThe Journal of cell biology, 2002
- De novo formation of centrosomes in vertebrate cells arrested during S phaseThe Journal of cell biology, 2002
- Loss of p53 and centrosome hyperamplificationOncogene, 2002
- Transient concentration of a γ‐tubulin‐related protein with a pericentrin‐related protein in the formation of basal bodies and flagella during the differentiation of Naegleria gruberiCell Motility, 2002
- Kinetics and regulation of de novo centriole assemblyCurrent Biology, 2001
- Centrosome-Dependent Exit of Cytokinesis in Animal CellsScience, 2001
- Papillomavirus infections — a major cause of human cancersBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1996
- Microsurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cellsCell, 1991
- De novo formation of centrioles in parthenogenetically activated, diploidized rabbit embryosBiology of the Cell, 1991